Therapeutic Applications of RNAi: Methods and Protocols / Edition 1

Therapeutic Applications of RNAi: Methods and Protocols / Edition 1

by John F. Reidhaar-Olson, Cristina M. Rondinone
     
 

ISBN-10: 1603272941

ISBN-13: 9781603272940

Pub. Date: 07/09/2009

Publisher: Springer-Verlag New York, LLC

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of

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Overview

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular BiologyT series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges

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Product Details

ISBN-13:
9781603272940
Publisher:
Springer-Verlag New York, LLC
Publication date:
07/09/2009
Series:
Methods in Molecular Biology Series, #555
Edition description:
2009
Pages:
148
Product dimensions:
7.60(w) x 10.40(h) x 0.60(d)

Table of Contents

1. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors

Zdravka Medarova, Mohanraja Kumar, Shu-wing Ng, and Anna Moore

2. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery

Muriel Golzio, Laurent Mazzolini, Aurélie Paganin-Gioanni, and Justin Teissié

3. Liposomal siRNA for Ovarian Cancer

Lingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, and Anil K. Sood

4. Therapeutic Applications of RNAi for Silencing Virus Replication

Ralph A. Tripp and Stephen Mark Tompkins

5. RNAi in the Malaria Vector, Anopheles gambiae

Flaminia Catteruccia and Elena A. Levashina

6. RNAi Using a Chitosan/siRNA Nanoparticle System: in vitro and in vivo Applications

Morten Østergaard Andersen, Kenneth Alan Howard, and Jørgen Kjems

7. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease through RNAi

Chris Towne and Patrick Aebischer

8. Studying Autoimmunity by in vivo RNA Interference

Stephan Kissler

9. Transcriptional Gene Silencing Using Small RNAs

Daniel H. Kim and John J. Rossi

10. Alternative Splicing as a Therapeutic Target for Human Diseases

Kenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, and Rajesh K. Gaur

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