Thin Cell Layer Culture System: Regeneration and Transformation Applications / Edition 1

Thin Cell Layer Culture System: Regeneration and Transformation Applications / Edition 1

by Duong Tan Nhut
     
 

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical

See more details below

Overview

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry.

The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants.

This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

Read More

Product Details

ISBN-13:
9781402012075
Publisher:
Springer Netherlands
Publication date:
03/31/2003
Edition description:
2003
Pages:
517
Product dimensions:
9.21(w) x 6.14(h) x 1.19(d)

Table of Contents

Preface
Abbreviation
Ch. 1Thin Cell Layer Concept1
Ch. 2Tobacco Thin Cell Layer morphogenesis17
Ch. 3Cells: Functional units of Thin Cell Layers65
Ch. 4Control of plant organogenesis: genetic and biochemical signals in plant organ form and development135
Ch. 5Methyl jasmonate and ethylene interfere with polyamine metabolism and organogenesis in tobacco Thin Cell Layers191
Ch. 6Biochemical and molecular markers in programmed plant differentiation and manipulation of the morphogenetic pathways in tobacco and lily by using Thin Cell Layer technique217
Ch. 7Thin Cell Layer morphogenesis as a powerful tool in ornamental plant micropropagation and biotechnology247
Ch. 8Thin Cell Layers and floral morphogenesis, floral genetics and in vitro flowering285
Ch. 9Regeneration and transformation of Lilium longiflorum by using Thin Cell Layer procedures343
Ch. 10Thin Cell Layer studies of vegetable, leguminous and medicinal plants387
Ch. 11Organogenesis of cereals and grasses by using Thin Cell Layer technique427
Ch. 12Thin Cell Layer technology in fruit crop regeneration451
Ch. 13Woody plant micropropagation and morphogenesis by Thin Cell Layers473
Ch. 14Genetic transformation using Thin Cell Layer495
Index of species513
Index of subject515

Read More

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >