Thyroid Hormone Receptors: Methods and Protocols

by Aria Baniahmad (Editor)

Overview

A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.

Product Details

ISBN-13:	9780896039957
Publisher:	Springer-Verlag New York, LLC
Publication date:	06/04/2002
Series:	Methods in Molecular Biology , #202
Edition description:	2002
Pages:	223
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

to Thyroid Hormone Receptors.- Null Mutant Mice for Thyroid Hormone Receptors.- Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray.- The Role of Thyroid Hormone Receptors in the Heart.- Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization.- The v-erbA Oncogene.- Gene Regulation by Thyroid Hormone Receptors.- Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome.- Function of Thyroid Hormone Receptors During Amphibian Development.- Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin.- Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex.

Editorial Reviews

Eleven essays by 22 international specialists discuss recent thyroid hormone receptors research. Among the topics covered: heart rate analysis, amphibian morphogenesis, transcriptional regulation by TRs in cell-free systems and in living cells, the isolation of TR- regulated protein complexes, the functional role of TRs in vertebrates, and the analysis of the oncogene v- in blood cell differentiation. Methods described include the generation of knockout mice, transgenic mice, transgenic and its use in molecular and chromatic-level analysis, microarray chip analysis, hybridization, the in vitro transcription system, chromatin immunoprecipitation, baculovirus expression system, and DNA transfer methods for cells and cell lines. For endocrinologists, geneticists, biochemists, and research physicians. Annotation c. Book News, Inc., Portland, OR (booknews.com)