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Tissue, Cell and Organ Engineering / Edition 1

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This volume collects the knowledge on how to bring nanomaterials and strategies together for assembling functional and structural artificial tissues. Treating tissue engineering in a materials science context—from nanomaterials to functional tissues—the text covers strategies, technologies and biological effects, drawing on a wide range of material types, including organic and inorganic materials, fibrous polymer scaffolds, nanocrystals, magnetic nanoparticles, nanotubes and nanowires.
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Editorial Reviews

From the Publisher
"Overall, this very well presented text successfully sets out the potential of nanotechnology in tissue engineering...The book serves as a very good introduction to a rapidly developing and exciting field."
Advanced Materials
Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This ninth volume in the Nanotechnologies for the Life Sciences reviews methods for the creation of nanomaterials and details the response of mammalian cells to various man-made objects, including nanoparticles, nanotubes, and nanofibers. The book's 12 chapters cover topics ranging from electrospinning technology for nanofibrous scaffolds in tissue engineering to nanoparticles and nanowires for cellular engineering. The reader enters a nanoworld at the atomic scale. Here we step across a DNA strand or meander along the folds in a globular protein. From this perspective, the extracellular matrices that surround cells appear as coarse fibrous meshes — scaffolds for the display of growth factors and adhesion molecules. Research in nanobiotechnology is aimed at engineering artificial scaffolds to support cellular organization and metabolism. The primary focus of this book is on biochemistry, bioengineering, and materials science.
Purpose: According to the editor, this book "can be considered as an encyclopedia on nanotechnological approaches to tissue, cell and organ engineering." The long-term goals of this expanding field are to provide replacement parts for nonfunctional tissues and organs. The authors believe that this book "will be a knowledge base for further advances that are bound to take place in the near future.
Audience: It is written for basic science and clinical researchers interested in biochemistry, bioengineering, and regenerative medicine. Researchers in chemistry and materials science will also appreciate the complexity and comprehensive nature of these excellent reviews. The editor thanks the reader "who has taken the time to join this journey."
Features: Each chapter provides a summary, introduction, detailed discussion, and complete references. The authors discuss, compare, illustrate, and review the literature. They provide examples of interesting results from their laboratories. The chapters are written by experts and the book is carefully edited. The drawings and continuous tone photomicrographs are interspersed with beautiful, full-color photomicrographs. The figures nicely illustrate the effects of novel substrates and culture conditions on the growth and differentiation of mammalian cells in culture.
Assessment: The value of this book hinges on whether artificial scaffolds (with appropriate stem cells added) will be useful for constructing artificial tissues and organs. A different approach is to let stem cells use existing biological matrices within tissues to regenerate biological structure. The two strategies (i.e., natural versus man-made template) are complementary. It remains to be seen which approach will have the most direct clinical application. The authors could have spent more time discussing their overall approach for adding back specific growth factors and adhesion molecules. What are the preferred coupling mechanisms? How will these issues be solved? This book is an excellent source of information for all those interested in applying engineering concepts to the field of regenerative medicine, with focus on new nanomaterials being investigated for use in tissue, cell, and organ engineering.
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Product Details

  • ISBN-13: 9783527313891
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 1/2/2007
  • Series: Nanotechnologies for the Life Sciences Series , #4
  • Edition number: 1
  • Pages: 540
  • Product dimensions: 6.97 (w) x 9.69 (h) x 1.23 (d)

Meet the Author

Challa Kumar is currently the Group Leader of Nanofabrication at the Center for Advanced Microstructures and Devices (CAMD), Baton Rouge, USA. His research interests are in developing novel synthetic methods for functional nanomaterials and innovative therapeutic, diagnostic and sensory tools based on nanotechnology. He has eight years of industrial R&D experience working for Imperial Chemical Industries and United Breweries prior to joining CAMD. He is the founding Editor-in-Chief of the Journal of Biomedical Nanotechnology, an international peer reviewed journal published by American Scientific Publishers, and the series editor for the ten-volume book series Nanotechnologies for the Life Sciences (NtLS) published by Wiley-VCH. He worked at the Max Planck Institute for Biochemistry in Munich, Germany, as a post doctoral fellow and at the Max Planck Institute for Carbon Research in M?, Germany, as an invited scientist. He obtained his Ph.D. degree in synthetic organic chemistry from Sri Sathya Sai Institute of Higher Learning, Prashanti Nilayam, India.
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Table of Contents


List of Authors.

1 Nanotechnology and Tissue Engineering: The Scaffold Based Approach (Lakshmi S. Nair, Subhabrata Bhattacharyya, and Cato T. Laurencin).

1.1 Overview.

1.2 Introduction.

1.3 The Importance of Scaffolds in Tissue Engineering.

1.4 Structure and Functions of Natural Extracellular Matrix.

1.5 Applications of Nanotechnology in Developing Scaffolds for Tissue Engineering.

1.6 Cell Behavior Towards Nano-based Matrices.

1.7 Applications of Nano-based Matrices as Scaffolds for Tissue Engineering.

1.8 Conclusions.


2 Polymeric Nanofibers in Tissue Engineering (Seow Hoon Saw, Karen Wang, Thomas Yong, and Seeram Ramakrishna).

2.1 Overview.

2.2 Introduction.

2.3 Classification of Nanofibers.

2.4 Nanofiber Fabrication.

2.5 Degradation and Absorption Kinetics of Nanofiber Scaffolds Compared with Conventional Scaffolds.

2.6 Advantages and Disadvantages of Nanofiber Scaffolds Compared with Other Conventional Scaffolds.

2.7 Biocompatibility of Nano-structured Tissue Engineered Implants.

2.8 Applications of Polymeric Nanofibers in Tissue Engineering.

2.9 Innovations in Nanofiber Scaffolds.

2.10 Conclusion.


3 Electrospinning Technology for Nanofibrous Scaffolds in Tissue Engineering (Wan-Ju Li, Rabie M. Shanti, and Rocky S. Tuan).

3.1 Introduction.

3.2 Nanofibrous Scaffolds.

3.3 Current Development of Electrospun Nanofibrous Scaffolds in Tissue Engineering.

3.4 Current Challenges and Future Directions.

3.5 Conclusion.


4 Nanofibrous Scaffolds and their Biological Effects (Laura A. Smith, Jonathan A. Beck, and Peter X. Ma).

4.1 Overview.

4.2 Introduction.

4.3 Methods of Formation.

4.4 Nanofibrous Composite Scaffolds.

4.5 Biological Effects of Nanofibers.

4.6 Tissue Formation.

4.7 Conclusion.


5 Nanophase Biomaterials for Tissue Engineering (Ramalingam Murugan and Seeram Ramakrishna).

5.1 Introduction: Problems with Current Therapies.

5.2 Tissue Engineering: A Potential Solution.

5.3 Stem Cells: The Essentials.

5.4 Nanobiomaterials: A New Generation Scaffolding Material.

5.5 Nanofibrous Scaffold Processing: Current Scenarios.

5.6 Cell???Matrix (Scaffold) Interactions.

5.7 Concluding Remarks.





6 Orthopedic Tissue Engineering Using Nanomaterials (Michiko Sato and Thomas J. Webster).

6.1 Preface.

6.2 Introduction: Problems with Current Implants.

6.3 A Potential Solution: Nanotechnology.

6.4 Considerations and Future Directions.



7 Hydroxyapatite Nanocrystals as Bone Tissue Substitute (Norberto Roveri and Barbara Palazzo).

7.1 Overview.

7.2 Introduction.

7.3 Biogenic Hydroxyapatite: Bone and Teeth.

7.4 Biomimetic Hydroxyapatite: Porous and Substituted Apatites.

7.5 Biologically Inspired Hydroxyapatite: HA???Collagen Composites and Coatings.

7.6 Functionalized Hydroxyapatite: HA Nanocrystals ??? Bioactive Molecules.

7.7 Conclusion and Future Challenges.



8 Magnetic Nanoparticles for Tissue Engineering (Akira Ito and Hiroyuki Honda).

8.1 Introduction.

8.2 Mesenchymal Stem Cell Isolation and Expansion.

8.3 Mag-seeding.

8.4 Construction of 3D Tissue-like Structure.

8.5 Conclusion.


9 Applications and Implications of Single-walled Carbon Nanotubes in Tissue Engineering (Peter S. McFetridge and Matthias U. Nollert).

9.1 Introduction.

9.2 Electromagnetic Fields for Tissue Regeneration.

9.3 Tissue Engineering.

9.4 SWNT Preparation: Purification and Functionalization.

9.5 Specific Applications of Carbon Nanotubes in Tissue Engineering.

9.6 Conclusions.


10 Nanoparticles for Cell Engineering ??? A Radical Concept (Beverly A. Rzigalinski, Igor Danelisen, Elizabeth T. Strawn, Courtney A. Cohen, and Chengya Liang).

10.1 Introduction and Overview.

10.2 Free Radicals and Oxidative Stress.

10.3 A Nanotechnological Approach to Oxidative Stress.

10.4 Nano-pharmacology.

10.5 Nanoparticle Antioxidants and Treatment of Disease.

10.6 Toxicology.

10.7 Summary.


11 Nanoparticles and Nanowires for Cellular Engineering (Jessica O. Winter).

11.1 Introduction.

11.2 Biological Opportunities at the Nanoscale.

11.3 Nanostructures to Modify Cell Adhesion and Migration.

11.4 Nanostructure Cellular Entry.

11.5 Intracellular Transport of Nanostructures.

11.6 Biomolecule Delivery Using Nanostructures.

11.7 Protein Manipulation.

11.8 Summary and Conclusions.


12 Nanoengineering of Biomaterial Surfaces (Ashwath Jayagopal and Venkatram Prasad Shastri).

12.1 Introduction.

12.2 Conventional Photolithography.

12.3 Electron-beam Lithography.

12.4 Soft Lithography.

12.5 Polymer-demixed Nanotopographies.

12.6 Star-shaped and other Novel Polymer Structures.

12.7 Vapor Deposition.

12.8 Self-assembly.

12.9 Particle Blasting.

12.10 Ion Beam and Plasma-guided Surface Engineering.

12.11 Sol???Gel Technology.

12.12 Nanolithography.

12.13 Laser-guided Strategies.

12.14 Rapid Prototyping Techniques.

12.15 Conclusions.




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