Transgenic Animal Technology: A Laboratory Handbook [NOOK Book]

Overview

Description
A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for ...
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Transgenic Animal Technology: A Laboratory Handbook

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Overview

Description
A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat.

Importance of Topic
One potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology.

Why This Title
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Contains a comprehensive collection of transgenic animal and gene transfer methods
Discusses background and introduction to techniques and animal systems
Teaches practical step-by-step protocols
New section on analysis

Audience: Students, postdoctoral fellows, and researchers at academic and industrial/agricultural laboratories using non-traditional transgenic models in such areas as molecular and cell biology, developmental biology, neuroscience, pharmacology, biophysiology, cardiology, oncology and genetics.

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Editorial Reviews

Booknews
A well-illustrated handbook that covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies employed by leading laboratories, and is suitable as a resource for researchers as well as a tool for training technicians and students. Metal comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher
"A ‘must have’ for every lab involved with transgenic technology."
-Andreas Kolb, Hannah Research Institute

"The handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained by reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques, which can be found by selection of specific chapters."-TRANSGENICS (2003)

"This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals."-ANALYTICAL BIOCHEMISTRY (2003)

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Product Details

  • ISBN-13: 9780080574806
  • Publisher: Elsevier Science
  • Publication date: 12/2/2012
  • Sold by: Barnes & Noble
  • Format: eBook
  • Edition number: 2
  • Pages: 618
  • File size: 18 MB
  • Note: This product may take a few minutes to download.

Meet the Author

Dr. Pinkert is an Associate Professor in the Department of Comparative Medicine and Director of the Transgenic Animal/ES Cell Resource at the University of Alabama at Birmingham. He earned his B.S. from Colorado State University, M.S. from Southern Illinois, and Ph.D. in 1983 from the University of Georgia, followed by postdoctoral training at the University of Pennsylvania. He was an Assistant Professor at the University of Missouri and then worked at DNX, Inc. (where his proposal for the National Transgenic Development Facility for the National Institute of Child Health and Human Development was awarded and implemented) before joining the UAB faculty. Dr. Pinkert's primary research efforts involve gene transfer, expression, and regulation using transgenic animal modeling. His work has illustrated the potential of modifying the immune system, enhancing growth performance, and the feasibility of molecular farming. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. He serves as an Editro of Transgenic Research and has served as an ad hoc reviewer or consultant for several journals, government agencies, and companies.
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Table of Contents

Section One
Overview.
Introduction to transgenic animals.

Section Two
Transgenic animal production focusing on the mouse model.
Transgenic animal production using DNA microinjection.
Factors affecting transgenic animal production.
Gene targeting in embryonic stem cells I: History and methodology.
Gene targeting in embryonic stem cells II. Conditional technologies.
Retrovirus-mediated gene transfer.
Nuclear transfer technologies.

Section Three
Production of transgenic laboratory and domestic animal species.
Production of transgenic rats.
Production of transgenic rabbits.
Production of transgenic fish.
Production of transgenic poultry.
Production of transgenic swine by DNA Microinjection.
Production of transgenic ruminants by DNA Microinjection.
Production of transgenic non-human primates.
Retrovirus-mediated gene transfer into oocytes.

Section Four: Molecular biology, analyses and enabling technologies
Vector design for Transgene Expression.
Analysis of transgene integration.
PCR optimization for Detection of Transgenic Integration.
Evaluation of Transgene Expression.
Control of gene expression (from standard gain/loss of function to anti-sense to PNAs).
Enabling technologies:Cryopreservation of gametes.
Enabling technologies:Embryo culture considerations.
Databases, Internet Resources and Genetic Nomenclature.
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