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Transgenic Animals / Edition 1

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Overview

During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues.

Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics.

Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

Covers transgenic rats, gene transfer into goat embryos, DNA delivery by cytoplasmic injection, transgenic salmonids.

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Product Details

  • ISBN-13: 9789057020698
  • Publisher: CRC Press
  • Publication date: 5/22/1997
  • Edition number: 1
  • Pages: 592
  • Product dimensions: 8.30 (w) x 10.90 (h) x 1.00 (d)

Table of Contents

PART I: OVERVIEW
Overview

PART II: THE TECHNIQUES FOR GENE TRANSFER
Gene Transfer into Mammal Embryos
In Vitro Mammal Embryo Manipulation
Gene Transfer into Bird Embryos
Gene Transfer into Fish and Lower Vertebrates
Gene Transfer into Invertebrates
The Use of ES Cells for Gene Transfer and Gene Targeting
The Direct Gene Transfer into Somatic Cells of Animals

PART III: THE FATE OF MICROINJECTED DNA INTO MAMMAL EMBRYOS
The Integration and Modification of Foreign DNA
The Vectors for Transgenesis

PART IV: THE USE OF TRANSGENIC ANIMALS
The Study of Gene Functions
The Generation of Animal Models for Biomedical and Pharmaceutical Studies
The Preparation of Recombinant Proteins from Transgenic Animals
The Modification of Milk Composition
The Protection of Animals Against Diseases
Towards the Introduction of New Genetic Traits in Domestic Animals by Transgenesis

PART V: THE NEW PROBLEMS GENERATED BY TRANSGENIC ANIMALS

Index

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