Transgenic Microalgae as Green Cell Factories

by Rosa Leïn (Editor), Aurora Galvïn Cejudo (Editor), Emilio Fernïndez (Editor)

Overview

Microalgae have been largely cultured and commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, in contrast to the large number of genetically modified bacteria, yeast and even higher plants, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed.

In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.

Product Details

ISBN-13:	9781489991324
Publisher:	Springer New York
Publication date:	11/25/2014
Series:	Advances in Experimental Medicine and Biology
Edition description:	2007
Pages:	131
Product dimensions:	7.01(w) x 10.00(h) x 0.01(d)

Table of Contents

CONTENTS 1. NUCLEAR TRANSFORMATION OF EUKARYOTIC MICROALGAE: HISTORICAL OVERVIEW, ACHIEVEMENTS AND PROBLEMS ................................................ 1 Rosa León and Emilio Fernández Abstract ............................................................................................................................... 1 Introduction ......................................................................................................................... 1 Microalgae Groups Transformed ....................................................................................... 2 Methods for Microalgae Transformation .......................................................................... 4 Characteristics of the Transformation Process ................................................................. 6 DNA Constructions Used in Transformation .................................................................... 6 Difficulties for Stable Expression of the Transgenes ........................................................ 8 Concluding Remarks ........................................................................................................... 8 2. TRANSFORMATION OF CYANOBACTERIA......................................... 12 Agustín Vioque Abstract .............................................................................................................................. 12 Introduction ....................................................................................................................... 12 Transformation of Cyanobacteria .................................................................................... 13 Applications ....................................................................................................................... 14 3. MOLECULAR BIOLOGY AND THEBIOTECHNOLOGICAL POTENTIAL OF DIATOMS .............................................................. 23 Peter Kroth Abstract .............................................................................................................................. 23 Diatom Biology .................................................................................................................. 23 Genetic Manipulation of Diatoms .................................................................................... 25 Biochemistry of Diatoms and Technological Applications ............................................. 29 Synthesis of Fatty Acids .................................................................................................... 29 Biomineralization .............................................................................................................. 30 Concluding Remarks ......................................................................................................... 31 00LeonAEMB 9 8/13/07, 9:53 AM x Contents 4. TOOLS AND TECHNIQUES FOR CHLOROPLAST TRANSFORMATION OF CHLAMYDOMONAS........................... 34 Saul Purton Abstract .............................................................................................................................. 34 Introduction ....................................................................................................................... 34 Delivery of DNA into the Chloroplast Compartment .................................................... 36 Integration of Transforming DNA ................................................................................... 37 Polyploidy and the Problems of Heteroplasmy ............................................................... 39 Selection Strategies ....................................................