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Troubleshooting LC Systems: A Comprehensive Approach to Troubleshooting LC Equipment and Separations / Edition 1

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Overview

Over the last 15 years, high-performance liquid chromatogra­ phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers. With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw­ driver, soldering iron, and various wrenches as constant compan­ ions in the LC lab. Today, many instruments contain micropro­ cessors as powerful as those of mainframe computers of earlier days. With this technology has come a variety of self-diagnostic tools that allow the LC system to locate many of its own prob­ lems. Traditionally, well-honed LC troubleshooting skills have been a result of years of work at the bench. Today the LC system itself often can do a better job of troubleshooting than the operator can. Yet many of the problems of the past are still the major problems of today: air bubbles, check valves, detector lamps, and, of course, problems with the separation. An added pressure on the operator of today's LC system is that of productivity-the lab often cannot afford unnecessary downtime. This means that the operator has to be a troubleshooting expert, or has to have that expertise at his or her fingertips. The present book was written to provide this expertise in an easy-to-use format for users at all levels of experience.

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Editorial Reviews

Booknews
As a result of teaching short courses on high performance liquid chromatography (LC), the authors present a study of how the instrument operates and its preventive maintenance to help the practicing chromatographer avoid problems that routinely cause crises and unproductive downtime in the lab. A useful handbook. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780896031517
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/28/1989
  • Edition description: 1989
  • Edition number: 1
  • Pages: 523
  • Product dimensions: 7.20 (w) x 9.48 (h) x 1.33 (d)

Table of Contents

General Considerations. Logical Approaches to Troubleshooting. Separation Basics. Principles of Troubleshooting. Prevention of Problems. Individual HPLC Modules. Reservoirs and Degassing. Pumps. Tubing. Fittings. Injectors and Autosamplers. Columns. Detectors. Recorders and Data Systems.Troubleshooting the Separation plus Other Problems. Separation Problems: Band Tailing and Peak Distortion. Separation Problems: Other Changes in the Appearance of the Chromatogram. Problems in Quantitation. Other Problems. Index.

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