Two-Dimensional Electrophoresis Protocols / Edition 1

Two-Dimensional Electrophoresis Protocols / Edition 1

by David Sheehan
     
 

ISBN-10: 1588299376

ISBN-13: 9781588299376

Pub. Date: 04/01/2009

Publisher: Springer-Verlag New York, LLC

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers

…  See more details below

Overview

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists. Written for: Protein scientists, molecular and cellular biologists

Read More

Product Details

ISBN-13:
9781588299376
Publisher:
Springer-Verlag New York, LLC
Publication date:
04/01/2009
Series:
Methods in Molecular Biology Series, #519
Edition description:
2009
Pages:
546
Product dimensions:
7.60(w) x 10.24(h) x 0.05(d)

Table of Contents

Preface v

Contributors xi

Overview Chapters

1 Two-Dimensional Electrophoresis: An Overview Richard Smith 3

2 Solubilization of Proteins in 2DE: An Outline Thierry Rabilloud 19

3 Selection of pH Ranges in 2DE Mireille Starita-Geribaldi 31

4 Difficult Proteins Ben Herbert Elizabeth Harry 47

5 Organelle Proteomics Matthias Plöet;scher Bernhard Granvogl Veronika Reisinger Axel Masanek Lutz Andreas Eichacker 65

6 Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry Alexander Leitner Wolfgang Lindner 83

7 Immunoblotting 2DE Membranes Brian McDonagh 103

Protocols Chapters

8 Troubleshooting Image Analysis in 2DE Bettina Leväet;nen Åsa M. Wheelock 113

9 Analysis of Bacterial Proteins by 2DE Philip Cash Evelyn Argo 131

10 Proteomic Analysis of Caenorhabditis elegans Pan-Young Jeong Keun Na Mi-Jeong Jeong David Chitwood Yhong-Hee Shim Young-Ki Paik 145

11 Protein Extraction for 2DE Claus Zabel Joachim Klose 171

12 Analysis of Proteins from Marine Molluscs Suze Chora Maria João Bebianno Michèle Roméo 197

13 Preparation and Analysis of Plastid Proteomes by 2DE Anne von Zychlinski Wilhelm Gruissem 205

14 High-Resolution 2DE Katrin Marcus Cornelia Joppich Caroline May Kathy Pfeiffer Barbara Sitek Helmut Meyer Kai Stuehler 221

15 Blue Native-Gel Electrophoresis Proteomics Kelly Andringa Adrienne King Shannon Bailey 241

16 2DE for Proteome Analysis of Human Metaphase Chromosomes Kiichi Fukui Susumu Uchiyama 259

17 Microsomal Proteomics Diana M. Wong Khosrow Adeli 273

18 Prefractionation Using Microscale Solution IEF Won-A Joo David Speicher 291

19 Diagonal Electrophoresis forDetection of Protein Disulphide Bridges Brian McDonagh 305

20 High-Resolution Large-Gel 2DE Claus Zabel Joachim Klose 311

21 Silver Staining of Proteins in 2DE Gels Cécile Lelong Mireille Chevallet Sylvie Luche Thierry Rabilloud 339

22 Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach Rukhsana Sultana Tanea Reed D. Allan Butterfield 351

23 Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells Sarah L. Cuddihy James W. Baty Kristin K. Brown Christine C. Winterbourn Mark B. Hampton 363

24 Detection of Ubiquitination in 2DE Brian McDonagh 377

25 Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry Sarka Beranova-Giorgianni Dominic M. Desiderio Francesco Giorgianni 383

26 Detection of Protein Glutathionylation Elisabetta Gianazza Ivano Eberini Pietro Ghezzi 397

27 Activity-Based Protein Profiling of Protein Tyrosine Phosphatases Chad Walls Bo Zhou Zhong-Yin Zhang 417

28 Active Protease Mapping in 2DE Gels Zhenjun Zhao Pamela J. Russell 431

29 Two-Dimensional Difference Gel Electrophoresis Gert Van den Bergh 439

30 Protein Expression Profiling Brian P. Bradley Bose Kalampanayil Michael C. O'Neill 455

31 C-Terminal Sequence Analysis of 2DE-Separated Proteins Bart Samyn Kjell Sergeant Jozef Van Beeumen 469

32 Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry Kazuishi Kubota Toshiyuki Kosaka Kimihisa Ichikawa 483

33 De Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination Kjell Sergeant Jozef Van Beeumen Bart Samyn 495

34 Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis Mai-Loan Huynh Pamela Russell Bradley Walsh 507

35 Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations Patricia M. Palagi Frédérique Lisacek Ron D. Appel 515

36 Creating 2DE Databases for the World Wide Web Christine Hoogland Khaled Mostaguir Ron D. Appel 533

Index 541

Read More

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >