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Doody's Review ServiceReviewer: Robert Michael Sargis, M.D., Ph.D.(University of Chicago Medical Center)
Description: This is a compilation of techniques useful for the laboratory study of diabetes with step-by-step directions for how to perform the most relevant procedures. Chapters are written by authorities in their specific fields, resulting in a book that covers many of the most relevant and up-to-date methods for studying diabetes.
Purpose: The book sets out to provide the relevant background behind the key techniques used for the study of diabetes along with up-to-date protocols to facilitate the dissemination of these techniques to other laboratories. Given the explosion in diabetes rates throughout the world, this book arrives at an opportune time. To a great extent, the book meets its objective of compiling procedures relevant to the study of diabetes. However, the broad title should not be taken to mean that all diabetes-related laboratory techniques are covered. The book is especially strong in its coverage of techniques for studying beta cell physiology. Those interested in other aspects of diabetes research should review the table of contents to ensure that it includes the topics of most interest to them.
Audience: It is written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. Written by authors who are experts in the field and complemented by current, relevant references, this book is a credible source of information for those seeking experimental advice.
Features: A wide range of topics relevant to the study of diabetes is covered, from single cell RT-PCR to hyperinsulinemic-euglycemic clamps in mice to gene expression profiling. The greatest strength of the book is its compilation of techniques for the study of pancreatic beta cells. Other areas are also covered, but there are fewer relevant chapters for those studying non-pancreatic tissues and cell lines. Several informative chapters are of general relevance to those studying diabetes, including chapters on choosing the "right" mouse model as well as nutritional models of diabetes.
Assessment: This is an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher's bookshelf.