Type 2 Diabetes: Methods and Protocols / Edition 1

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Overview

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Prools, leading investigators provide up-to-date explanations of commonly used laboratory prools used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Prools offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

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Editorial Reviews

From The Critics
Reviewer: Robert Michael Sargis, M.D., Ph.D.(University of Chicago Medical Center)
Description: This is a compilation of techniques useful for the laboratory study of diabetes with step-by-step directions for how to perform the most relevant procedures. Chapters are written by authorities in their specific fields, resulting in a book that covers many of the most relevant and up-to-date methods for studying diabetes.
Purpose: The book sets out to provide the relevant background behind the key techniques used for the study of diabetes along with up-to-date protocols to facilitate the dissemination of these techniques to other laboratories. Given the explosion in diabetes rates throughout the world, this book arrives at an opportune time. To a great extent, the book meets its objective of compiling procedures relevant to the study of diabetes. However, the broad title should not be taken to mean that all diabetes-related laboratory techniques are covered. The book is especially strong in its coverage of techniques for studying beta cell physiology. Those interested in other aspects of diabetes research should review the table of contents to ensure that it includes the topics of most interest to them.
Audience: It is written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. Written by authors who are experts in the field and complemented by current, relevant references, this book is a credible source of information for those seeking experimental advice.
Features: A wide range of topics relevant to the study of diabetes is covered, from single cell RT-PCR to hyperinsulinemic-euglycemic clamps in mice to gene expression profiling. The greatest strength of the book is its compilation of techniques for the study of pancreatic beta cells. Other areas are also covered, but there are fewer relevant chapters for those studying non-pancreatic tissues and cell lines. Several informative chapters are of general relevance to those studying diabetes, including chapters on choosing the "right" mouse model as well as nutritional models of diabetes.
Assessment: This is an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher's bookshelf.
From The Critics
Reviewer:Robert Michael Sargis, M.D., Ph.D.(University of Chicago Medical Center)
Description:This is a compilation of techniques useful for the laboratory study of diabetes with step-by-step directions for how to perform the most relevant procedures. Chapters are written by authorities in their specific fields, resulting in a book that covers many of the most relevant and up-to-date methods for studying diabetes.
Purpose:The book sets out to provide the relevant background behind the key techniques used for the study of diabetes along with up-to-date protocols to facilitate the dissemination of these techniques to other laboratories. Given the explosion in diabetes rates throughout the world, this book arrives at an opportune time. To a great extent, the book meets its objective of compiling procedures relevant to the study of diabetes. However, the broad title should not be taken to mean that all diabetes-related laboratory techniques are covered. The book is especially strong in its coverage of techniques for studying beta cell physiology. Those interested in other aspects of diabetes research should review the table of contents to ensure that it includes the topics of most interest to them.
Audience:It is written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. Written by authors who are experts in the field and complemented by current, relevant references, this book is a credible source of information for those seeking experimental advice.
Features:A wide range of topics relevant to the study of diabetes is covered, from single cell RT-PCR to hyperinsulinemic-euglycemic clamps in mice to gene expression profiling. The greatest strength of the book is its compilation of techniques for the study of pancreatic beta cells. Other areas are also covered, but there are fewer relevant chapters for those studying non-pancreatic tissues and cell lines. Several informative chapters are of general relevance to those studying diabetes, including chapters on choosing the "right" mouse model as well as nutritional models of diabetes.
Assessment:This is an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher's bookshelf.
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Product Details

  • ISBN-13: 9781934115152
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/16/2009
  • Series: Methods in Molecular Biology Series , #560
  • Edition description: 2009
  • Edition number: 1
  • Pages: 278
  • Product dimensions: 7.50 (w) x 10.40 (h) x 0.70 (d)

Table of Contents

1) Selecting the 'Right' Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research
Edward H. Leiter

2) Nutritional Models of Type 2 Diabetes Mellitus
Beverly Sara Muhlhausler

3) The Isolation and Purification of Rodent Pancreatic Islets of Langerhans
Jacqueline F. O’Dowd

4) The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans
Anna L. Nolan and Jacqueline F. O’Dowd

5) The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ß-Cell Lines
Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell

6) In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function
Anna L. Nolan

7) Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells
Dany Muller, Peter M. Jones, and Shanta J. Persaud

8) Laser Capture Microdissection of Human Pancreatic b –Cells and RNA Preparation for Gene Expression Profiling
Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, and Gordon C. Weir

9) In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells
Wan-chun Li

10) The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes
Nicky Konstantopoulos and Juan Carlos Molero-Navajas

11) Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell Size
Eduard Montanya and Noèlia Téllez

12) Morphology of Pancreatic Islets: A Time Course of Pre-Diabetes in Zucker Fatty Rats
Petra Augstein and Eckhard Salzsieder

13) Fluorescent Immunohishemistry and in situ Hybridization Analysis of Pancreas
Xiuli Wang, Shundi Ge, and Gay M. Crooks

14) The Measurement of Insulin Secretion Using Pancreas Perfusionin the Rodent
Edward T. Wargent

15) Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo
Jason K. Kim

16) Gene Expression Analysis in Diabetes Research
Peter White and Klaus H. Kaestner

17) Gene Expression Mining in Type 2 Diabetes Research
Donald R. Dunbar

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