Uncovering the origins of developmental hematopoiesis lessons from the Ncx1(-/-) mouse embryo.

More About This Textbook

Overview

The yolk sac is accepted as the lone site of primitive hematopoiesis. The role of the yolk sac (YS) in generating definitive hematopoietic progenitors, however, has remained controversial as they are detected coincident with the initiation of the first heartbeat. The Ncx1-/- embryo fails to initiate a heartbeat and yet continues to develop for nearly 48 hours. To assure that the lack of circulation had no direct effect on hematopoiesis, we conducted LacZ staining, RT-PCR, annexin V apoptosis analysis, cell counting, zeta-globin in-situ hybridization, and benzidine staining. We have determined that the Ncx1-/- embryo is an appropriate model for the analysis of the temporal and spatial distribution of hematopoietic progenitor cells in an embryonic environment lacking circulation. Few or no hematopoietic progenitors are found in the Ncx1-/- PSp as late as E10, resulting in a 103-fold enrichment of progenitors in the Ncx1 -/- YS. We conclude that the PSp does not appear to function as a site of active hematopoiesis prior to E10. Furthermore, our findings support a model in which large numbers of definitive hematopoietic progenitors and primitive erythroblasts are generated in the YS between E8.0 and E10 and enter the embryo proper to seed the liver at the onset of embryonic circulation. The study of hematopoietic development in the Ncx1 -/- embryo was further expanded to examine placental hematopoiesis, fetal liver organ culture, colony forming units---spleen, as well as early lymphoid development. The results of these studies suggest that the placenta produces cells with hematopoietic potential de novo in the absence of systemic circulation, that embryonic hematopoietic progenitors can be expanded in hanging drop co-culture with fetal liver cells, that a quantification of CFU-S from Ncx1-/- embryos is possible with sufficient resources, and that there is evidence for the de novo emergence of a B lymphoid population in the YS.
Read More Show Less

Product Details

  • BN ID: 2940031989777
  • Publisher: ProQuest LLC
  • Sold by: Barnes & Noble
  • Format: eTextbook
  • Pages: 192

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)