The Use Of Carbon-13 Carbonyl Labeled Residues To Develop And Refine Site-Specific Nmr Secondary Structure Analysis Techniques.

Paperback (Print)
Not Available on

More About This Textbook


Synthetic 13C=O labeling of peptides provides a means by which NMR analysis elucidates chemical shifts that are indicative of site-specific secondary structure fold. The primary targets for analysis in this study are a helices and beta hairpins. The investigation into alpha helices involved alanine rich peptides, with 13C=O labeled alanines at many locations throughout the sequence, probing the effects of various residue point mutations at the N-terminus, middle and C-terminus of the helix. From these experiments, Lifson-Roig parameters were derived and applied to our helicity prediction algorithm Helix1.5. Additionally, the effects of various capping motifs (SEDE, Schellman and D-Arg) at different points along the length of the helix were probed using 13C=O labels. beta Hairpin studies involved 13C=O labeled valines incorporated into cross-strand bonding and nonbonding positions. The cross-strand bonded valines show an excellent correlation between 13C=O chemical shift deviations and fraction fold determined by 2D NMR, indicating that the chemical shift difference between outward non-bonded carbonyls and inward bonded carbonyls, in beta hairpins, is representative of 0 and 100 percent fold, whereby fraction fold can be determined with selectively placed 13C=O labels. The difference between outward non-bonded carbonyl and cross-strand bonded carbonyl chemical shift deviations in proteins was found by cross-referencing the PDB with the BMRB. The differences in proteins when compared to peptides are similar, but are statistically indistinguishable. The investigations performed in the course of my research establish 13C=O labeling as a simple, yet efficient site-specific NMR technique that can be used to probe secondary structure.
Read More Show Less

Product Details

  • ISBN-13: 9781244012530
  • Publisher: BiblioLabsII
  • Publication date: 9/11/2011
  • Pages: 202
  • Product dimensions: 7.44 (w) x 9.69 (h) x 0.43 (d)

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)