Vaccine Protocols / Edition 2

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This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.

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Editorial Reviews

From the Publisher
"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS

From reviews of the first edition...

"...cover[s] concepts and prools applicable to a wide range of vaccines and infectious diseases and cancer...brings together many prools of value to research and development scientists involved in the vaccine technology area."

-Immunology and Cell Biology

"...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation."

-Doody's Health Sciences Book Review Journal

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Product Details

  • ISBN-13: 9781617373824
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series, #87
  • Edition description: Softcover reprint of hardcover 2nd ed. 2003
  • Edition number: 2
  • Pages: 416
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.95 (d)

Table of Contents

Overview of Vaccines
Gordon Ada

Temperature-Sensitive Mutant Vaccines
Craig R. Pringle

Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus
Anthony R. Fooks

Live Viral Vectors: Vaccinia Virus
Caroline Staib and Gerd Sutter

Live Viral Vectors: Semliki Forest Virus
Gunilla B. Karlsson and Peter J. Liljeström

Development of Attenuated Salmonella Strains That Express Heterologous Antigens
Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Arcila, and Gordon Dougan

Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria
Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells

Synthetic Peptides
Michael J. Francis

Genetic Detoxification of Bacterial Toxins
Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli

Preparation of Polysaccharide-Conjugate Vaccines
Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman

Adjuvant Formulations for Experimental Vaccines
Duncan E. S. Stewart-Tull

Incorporation of Immunomodulators into Plasmid DNA Vaccines
Linda S. Klavinskis, Philip Hobson, and Andrew Woods

Microencapsulation of Vaccine Antigens
David H. Jones

Lyophilization of Vaccines: Current Trends
Gerald D. J. Adams

Stimulation of Mucosal Immunity
David J. M. Lewis and Christopher M. M. Hayward

Induction and Detection of T-Cell Responses
Kingston H. G. Mills

Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses
Rachel V. Samuel and Toma's Hanke

Assessment of Functional Antibody Responses
Ray Borrow and Paul Balmer

The Use of Complete Genome Sequences in VaccineDesign
Nigel J. Saunders and Sarah Butcher

Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment
Michael J. Dennis

Clinical Trials
Paddy Farrington and Elizabeth Miller

Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release
Elwyn Griffiths and Ivana Knezevic

DNA Vaccination: An Update
Douglas B. Lowrie

From Vaccine Research to Manufacture: A Guide for the Researcher
Nigel Allison and Howard S. Tranter


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