Vaccine Protocols / Edition 2

Vaccine Protocols / Edition 2

by Andrew P. Robinson, Martin P. Cranage, Michael J. Hudson
     
 

View All Available Formats & Editions

ISBN-10: 1617373826

ISBN-13: 9781617373824

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated

Overview

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.

Product Details

ISBN-13:
9781617373824
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Medicine Series , #87
Edition description:
Softcover reprint of hardcover 2nd ed. 2003
Pages:
416
Product dimensions:
5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Overview of Vaccines

Gordon Ada

Temperature-Sensitive Mutant Vaccines

Craig R. Pringle

Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus

Anthony R. Fooks

Live Viral Vectors: Vaccinia Virus

Caroline Staib and Gerd Sutter

Live Viral Vectors: Semliki Forest Virus

Gunilla B. Karlsson and Peter J. Liljeström

Development of Attenuated Salmonella Strains That Express Heterologous Antigens

Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Arcila, and Gordon Dougan

Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria

Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells

Synthetic Peptides

Michael J. Francis

Genetic Detoxification of Bacterial Toxins

Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli

Preparation of Polysaccharide-Conjugate Vaccines

Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman

Adjuvant Formulations for Experimental Vaccines

Duncan E. S. Stewart-Tull

Incorporation of Immunomodulators into Plasmid DNA Vaccines

Linda S. Klavinskis, Philip Hobson, and Andrew Woods

Microencapsulation of Vaccine Antigens

David H. Jones

Lyophilization of Vaccines: Current Trends

Gerald D. J. Adams

Stimulation of Mucosal Immunity

David J. M. Lewis and Christopher M. M. Hayward

Induction and Detection of T-Cell Responses

Kingston H. G. Mills

Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses

Rachel V. Samuel and Tomás Hanke

Assessment of Functional Antibody Responses

Ray Borrow and Paul Balmer

The Use of Complete Genome Sequences in Vaccine Design

Nigel J. Saunders and Sarah Butcher

Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment

Michael J. Dennis

Clinical Trials

Paddy Farrington and Elizabeth Miller

Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release

Elwyn Griffiths and Ivana Knezevic

DNA Vaccination: An Update

Douglas B. Lowrie

From Vaccine Research to Manufacture: A Guide for the Researcher

Nigel Allison and Howard S. Tranter

Index

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >