Vascular Biology Protocols / Edition 1

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Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.
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Editorial Reviews

Doody's Review Service
Reviewer: Vera P. Shively, BS, MS (Northwestern University Feinberg School of Medicine)
Description: This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models.
Purpose: The purpose is to provide a comprehensive manual for in vivo and in vitro techniques commonly used to study the progression of cardiovascular disease. A book such as this one was needed and it meets the authors' objectives.
Audience: Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. Although the book is written for beginners, even experienced researchers would find techniques that are new to them.
Features: The book includes protocols for animal models, ex vivo organ systems and relevant functional studies, cell culture isolations, and pertinent experiments. In addition, it presents protocols for basic as well as sophisticated histology, along with instructions for the assessment of results. The final chapter includes methodology for stem cell therapy. For researchers in vascular biology, the strength of this book is the relevance of the protocols and the detail in which they are described. The sections on materials and methods are the most comprehensive I've seen, including separate lists for solutions, supplies, and surgical tools, as well as extensive notes and references on the nuances of each technique. The protocols for animal surgery are particularly thorough. One of the shortcomings of the book is paradoxically one of its assets — the "see Notes" feature. Basically, it is an example of the limitations, and sometimes awkwardness, of print in the age of electronic text with links. The "see Notes" feature augments each chapter with relevant information, but it is a bit of a nuisance to flip back and forth through the pages.
Assessment: This would be a valuable addition to any laboratory doing research in vascular biology. It is small in size, but substantial in its usefulness to researchers in this area of study. It is not a replacement for the Current Protocols series from John Wiley & Sons, but could be considered a complement to this series as an initial resource for protocols with a narrower focus.
From the Publisher

From the reviews:

"This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. … Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. … This would be a valuable addition to any laboratory doing research in vascular biology." (Vera P. Shively, Doody’s Review Service, December, 2008)

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Product Details

  • ISBN-13: 9781588295743
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 7/25/2008
  • Series: Methods in Molecular Medicine Series, #139
  • Edition description: 2007
  • Edition number: 1
  • Pages: 372
  • Product dimensions: 6.00 (w) x 9.00 (h) x 0.80 (d)

Table of Contents

Preface v

Contributors ix

1 Rat Carotid Artery Balloon Injury Model David A. Tulis 1

2 Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model David A. Tulis 31

3 Plaque Rupture Model in Mice Takeshi Sasaki Kae Nakamura Masafumi Kuzuya 67

4 Immunostaining of Mouse Atherosclerotic Lesions Hong Lu Debra L. Rateri Alan Daugherty 77

5 Surgical Animal Model of Ventricular Hypertrophy Giuseppe Marano Alberto U. Ferrari 95

6 Animal Models of Hypertension Brett M. Mitchell Thomas Wallerath Ulrich Forstermann 105

7 Rat Models of Cardiac Insulin Resistance Sanjoy Ghosh Brian Rodrigues Jun Ren 113

8 The Isolated, Perfused Pseudo-Working Heart Model Gary J. Grover Rajni Singh 145

9 Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice Rajakumar V. Donthi Paul N. Epstein 151

10 Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress Camila Manrique Guido Lastra Javad Habibi Yongzhong Wei E. Matthew Morris Craig S. Stump James R. Sowers 163

11 Detection and Quantification of Apoptosis in the Vasculature Wendy A. Boivin David J. Granville 181

12 Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction Keith R. Brunt Sean R. R. Hall Christopher A. Ward Luis G. Melo 197

13 Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells Christopher J. Boos Andrew D. Blann Gregory Y. H. Lip 211

14 Evaluation of Endothelial Function by In Vivo Microscopy Rosario Scalia 225

15 Haptotaxis of Endothelial Cell Migration Under Flow Steve Hsu Rahul Thakar Song Li 237

16 Isolation, Culture, and Functional Analysis ofAdult Mouse Cardiomyocytes Ronglih Liao Mohit Jain 251

17 Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes Loren E. Wold Jun Ren 263

18 Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia Kelly R. Pitts Christopher F. Toombs 271

19 Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells Nair Sreejayan Xiaoping Yang 283

20 Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues Hua Cai Sergey Dikalov Kathy K. Griendling David G. Harrison 293

21 Assessment of Protein Glycoxidation in Ventricular Tissues Shi-Yan Li Jun Ren 313

22 Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes Takashi Matsui Amy J. Davidoff 329

23 Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research Angel Garcia 339

24 Stem Cell Therapy in the Heart and Vasculature Loren E. Wold Wangde Dai Joan S. Dow Robert A. Kloner 355

Index 367

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