Vascular Biology Protocols / Edition 1

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Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.

Comprehensive and easy-to-use, Vascular Biology Protocols will prove valuable for all those who wish to broaden their knowledge of cardiovascular research tools.

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Editorial Reviews

From the Publisher

From the reviews:

"This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. … Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. … This would be a valuable addition to any laboratory doing research in vascular biology." (Vera P. Shively, Doody’s Review Service, December, 2008)

Doody's Review Service
Reviewer: Vera P. Shively, BS, MS (Northwestern University Feinberg School of Medicine)
Description: This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models.
Purpose: The purpose is to provide a comprehensive manual for in vivo and in vitro techniques commonly used to study the progression of cardiovascular disease. A book such as this one was needed and it meets the authors' objectives.
Audience: Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. Although the book is written for beginners, even experienced researchers would find techniques that are new to them.
Features: The book includes protocols for animal models, ex vivo organ systems and relevant functional studies, cell culture isolations, and pertinent experiments. In addition, it presents protocols for basic as well as sophisticated histology, along with instructions for the assessment of results. The final chapter includes methodology for stem cell therapy. For researchers in vascular biology, the strength of this book is the relevance of the protocols and the detail in which they are described. The sections on materials and methods are the most comprehensive I've seen, including separate lists for solutions, supplies, and surgical tools, as well as extensive notes and references on the nuances of each technique. The protocols for animal surgery are particularly thorough. One of the shortcomings of the book is paradoxically one of its assets — the "see Notes" feature. Basically, it is an example of the limitations, and sometimes awkwardness, of print in the age of electronic text with links. The "see Notes" feature augments each chapter with relevant information, but it is a bit of a nuisance to flip back and forth through the pages.
Assessment: This would be a valuable addition to any laboratory doing research in vascular biology. It is small in size, but substantial in its usefulness to researchers in this area of study. It is not a replacement for the Current Protocols series from John Wiley & Sons, but could be considered a complement to this series as an initial resource for protocols with a narrower focus.
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Product Details

  • ISBN-13: 9781617376504
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/22/2014
  • Series: Methods in Molecular Medicine Series , #139
  • Edition description: 2007
  • Edition number: 1
  • Pages: 372

Table of Contents

Table of Contents Preface Contributors Rat Carotid Artery Balloon Injury Model David A. Tulis Histological and Morphometric Analyses for Rat Carotid Artery Balloon Injury David A. Tulis Plaque Rupture Model in Mice Takeshi Sasaki, Kae Nakamura and Masafumi Kuzuya Immunostaining of Mouse Atherosclerotic Lesions Hong Lu, Debra L. Rateri and Alan Daugherty Surgical Animal Model of Ventricular Hypertrophy Giuseppe Marano and Alberto U. Ferrari Animal Models of Hypertension Brett M. Mitchell, Thomas Wallerath and Ulrich Förstermann Rat Models of Cardiac Insulin Resistance Sanjoy Ghosh, Brian Rodrigues and Jun Ren The Isolated, Perfused Pseudo-Working Heart Model Gary J. Grover and Rajni Singh Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice Rajakumar V. Donthi and Paul N. Epstein Methods in Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress Camila Manrique, Guido Lastra, Javad Habibi, Yongzhong Wei, E. Matthew Morris, Craig S. Stump and James R. Sowers Detection and Quantification of Apoptosis in the Vasculature Wendy A. Boivin and David J. Granville Endothelial Progenitor Cells and Mesenchymal Stem Cell Isolation, Characterization and Viral Transduction Keith R. Brunt, Sean RR Hall, Christopher A. Ward and Luis G. Melo Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells Christopher J Boos, Andrew D. Blann and Gregory Y. H. Lip Evaluation of Endothelial Function by In-VivoMicroscopy Rosario Scalia Haptotaxis of Endothelial Cell Migration under Flow Steve Hsu, Rahul Thakar, and Song Li Isolation Culture and Functional Analysis of Adult Mouse Cardiomyocytes Ronglih Liao and Mohit Jain Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes Loren E. Wold and Jun Ren Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia Kelly R. Pitts and Christopher F. Toombs Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells Nair Sreejayan and Xiaoping Yang Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues: Comparison of Sensitivity and Specificity. Hua Cai, Sergey Dikalov, Kathy K. Griendling and David G. Harrison Assessment of Protein Glycoxidation in Ventricular Tissues Shi-Yan Li and Jun Ren Assessment of PI-3 kinase and Akt in Ischemic Heart Diseases in Diabetes Takashi Matsui and Amy J. Davidoff Two-dimensional Gel Electrophoresis in Platelet Proteomics Research Ángel García-Alonso Stem Cell Therapy in the Heart and Vasculature Loren E. Wold, Wangde Dai, Joan S. Dow and Robert A. Kloner
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