Virus Culture: A Practical Approach

Virus Culture: A Practical Approach

by Alan J. Cann, A. J. Cann
     
 

ISBN-10: 0199637156

ISBN-13: 9780199637157

Pub. Date: 05/28/2000

Publisher: Oxford University Press, USA

Virus Culture: A Practical Approach provides a broad treatment of the principles and practice of virus culture and will be of interest to all those, whether in academic, industrial, or clinical research, involved in virus culture. The first chapter is an overview of cell culture techniques essential for virologists. Other techniques then covered are isolating,

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Overview

Virus Culture: A Practical Approach provides a broad treatment of the principles and practice of virus culture and will be of interest to all those, whether in academic, industrial, or clinical research, involved in virus culture. The first chapter is an overview of cell culture techniques essential for virologists. Other techniques then covered are isolating, identifying, concentrating, and purifying viruses. Electron Microscopy as applied to virology is also explained. Chapter 6 is about creating virus vaccines and chapters 7 and 8 cover antiserum production, monoclonal antibodies and antiviral drug testing. The final chapter describes the methods used to study plant viruses.

Product Details

ISBN-13:
9780199637157
Publisher:
Oxford University Press, USA
Publication date:
05/28/2000
Series:
Practical Approach Series, #208
Pages:
296
Product dimensions:
6.40(w) x 9.30(h) x 0.90(d)

Table of Contents

List of Contributors
Abbreviations
1Cell culture1
1Introduction1
2Culture media5
3Good practice in cell culture7
4Principles of cell banking8
5Subculture of cells9
6Quantification of cell cultures11
7Cryopreservation and storage of cell lines13
8Quality control testing of cell lines16
9Transportation30
2Virus isolation33
1Why culture viruses?33
2Viruses not amenable to cultivation35
3Treatment of clinical specimens for virus isolation41
4Identification of virus replication45
5Passaging of viruses46
6Confirmation of virus isolation48
7Adaptations of cell culture52
8Conclusions59
3Concentration and purification of viruses61
1The need for virus concentration and purification61
2Virus concentration: general principles64
3Purification of virus: identification of virus-containing fractions67
4Purification by ultracentrifugation68
5Assessment of purity78
4Assays for virus infection81
1Introduction81
2Identification of viruses isolated in cell culture81
3Virus antigen detection88
4Virus genome detection91
5Detection of specific anti-viral antibodies100
5Electron microscopy of viruses111
1Introduction111
2Virus structure and strategies for virus detection118
3Specimen preparation118
4EM grids and support films123
5Negative staining124
6Embedding and thin sectioning132
7Immuno-electron microscopy (IEM)138
8Limitations of TEM in cell culture146
9Safety precautions146
6Virus vaccines149
1Introduction149
2Non-living virus vaccines152
3Live, attenuated virus vaccines156
4Delivery of vaccine antigens through vector technology and live, recombinant micro-organisms161
5DNA vaccines164
6Replication-deficient viruses as vaccines167
7Enhancement strategies for virus vaccines169
8In vitro and in vivo models in vaccine development173
9Control and licensing of virus vaccines175
10Future developments179
7Antiserum production and monoclonal antibodies183
1Introduction183
2Antigen preparation183
3Production of antisera185
4Production of monoclonal antibodies187
5Isotyping199
6Purification199
8Antiviral drug testing201
1Introduction201
2The role of cell culture in testing antiviral compounds203
3The design of the anti-viral testing laboratory205
4Testing procedures209
5Animal model infections223
6Genetic analysis of virus resistance to antiviral drugs232
7Conclusions235
9Plant virus culture239
1Introduction239
2Isolation of plant viruses241
3Tissue culture and plant viruses252
4Assay of plant viruses260
App. 1Suppliers of specialist items267
Index271

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