Virus Culture: A Practical Approach

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Overview


Virus Culture: A Practical Approach provides a broad treatment of the principles and practice of virus culture and will be of interest to all those, whether in academic, industrial, or clinical research, involved in virus culture. The first chapter is an overview of cell culture techniques essential for virologists. Other techniques then covered are isolating, identifying, concentrating, and purifying viruses. Electron Microscopy as applied to virology is also explained. Chapter 6 is about creating virus vaccines and chapters 7 and 8 cover antiserum production, monoclonal antibodies and antiviral drug testing. The final chapter describes the methods used to study plant viruses.

The book contains black-and-white illustrations.

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Editorial Reviews

Booknews
Not the next stage in learning how to deal with people from a different culture, but a guide to growing viruses in order to conduct research in virology. Mostly British, with a German and a few South African, scientists detail the procedures for isolating, identifying, concentrating, and purifying viruses. They also describe electron microscopy as applied to virology, the production of virus vaccines and antiserum, and testing antiviral drugs. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher

"Not the next stage in learning how to deal with people from a different culture, but a guide to growing viruses in order to conduct research in virology. Mostly British, with a German and a few South African, scientists detail the procedures for isolating, identifying, concentrating, and purifying viruses. They also describe electron microscopy as applied to virology, the production of virus vaccines and antiserum, and testing antiviral drugs."--SciTech Book News

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Product Details

  • ISBN-13: 9780199637157
  • Publisher: Oxford University Press, USA
  • Publication date: 5/28/2000
  • Series: Practical Approach Series , #208
  • Pages: 296
  • Product dimensions: 6.40 (w) x 9.30 (h) x 0.90 (d)

Table of Contents

List of Contributors
Abbreviations
1 Cell culture 1
1 Introduction 1
2 Culture media 5
3 Good practice in cell culture 7
4 Principles of cell banking 8
5 Subculture of cells 9
6 Quantification of cell cultures 11
7 Cryopreservation and storage of cell lines 13
8 Quality control testing of cell lines 16
9 Transportation 30
2 Virus isolation 33
1 Why culture viruses? 33
2 Viruses not amenable to cultivation 35
3 Treatment of clinical specimens for virus isolation 41
4 Identification of virus replication 45
5 Passaging of viruses 46
6 Confirmation of virus isolation 48
7 Adaptations of cell culture 52
8 Conclusions 59
3 Concentration and purification of viruses 61
1 The need for virus concentration and purification 61
2 Virus concentration: general principles 64
3 Purification of virus: identification of virus-containing fractions 67
4 Purification by ultracentrifugation 68
5 Assessment of purity 78
4 Assays for virus infection 81
1 Introduction 81
2 Identification of viruses isolated in cell culture 81
3 Virus antigen detection 88
4 Virus genome detection 91
5 Detection of specific anti-viral antibodies 100
5 Electron microscopy of viruses 111
1 Introduction 111
2 Virus structure and strategies for virus detection 118
3 Specimen preparation 118
4 EM grids and support films 123
5 Negative staining 124
6 Embedding and thin sectioning 132
7 Immuno-electron microscopy (IEM) 138
8 Limitations of TEM in cell culture 146
9 Safety precautions 146
6 Virus vaccines 149
1 Introduction 149
2 Non-living virus vaccines 152
3 Live, attenuated virus vaccines 156
4 Delivery of vaccine antigens through vector technology and live, recombinant micro-organisms 161
5 DNA vaccines 164
6 Replication-deficient viruses as vaccines 167
7 Enhancement strategies for virus vaccines 169
8 In vitro and in vivo models in vaccine development 173
9 Control and licensing of virus vaccines 175
10 Future developments 179
7 Antiserum production and monoclonal antibodies 183
1 Introduction 183
2 Antigen preparation 183
3 Production of antisera 185
4 Production of monoclonal antibodies 187
5 Isotyping 199
6 Purification 199
8 Antiviral drug testing 201
1 Introduction 201
2 The role of cell culture in testing antiviral compounds 203
3 The design of the anti-viral testing laboratory 205
4 Testing procedures 209
5 Animal model infections 223
6 Genetic analysis of virus resistance to antiviral drugs 232
7 Conclusions 235
9 Plant virus culture 239
1 Introduction 239
2 Isolation of plant viruses 241
3 Tissue culture and plant viruses 252
4 Assay of plant viruses 260
App. 1 Suppliers of specialist items 267
Index 271
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