Virus-Host Interactions: Methods and Protocols

Overview

Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein interactions, as well as cell biological and immunological methodologies. Viruses represent a reduced form of life that depends on host cells for propagation. To this end, viruses approach and penetrate cells and ...

See more details below
Hardcover (2013)
$139.00
BN.com price
Other sellers (Hardcover)
  • All (6) from $100.89   
  • New (6) from $100.89   
Sending request ...

Overview

Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein interactions, as well as cell biological and immunological methodologies. Viruses represent a reduced form of life that depends on host cells for propagation. To this end, viruses approach and penetrate cells and usurp cellular machineries for their own benefit. Recent technological improvements have enabled the systematic analysis of the virus-host interplay be it on the genomic, the transcriptomic, or proteomic level. In parallel, bioinformatic tools have emerged in support of the large datasets generated by these high-throughput approaches. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Virus-Host Interactions: Methods and Protocols will prove invaluable to professionals and novices with its well-honed methodologies and protocols.

Read More Show Less

Product Details

  • ISBN-13: 9781627036009
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/11/2013
  • Series: Methods in Molecular Biology Series , #1064
  • Edition description: 2013
  • Edition number: 1
  • Pages: 372
  • Product dimensions: 6.90 (w) x 10.00 (h) x 1.00 (d)

Table of Contents

1. A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions

Hannah Striebinger, Manfred Kögl, and Susanne M. Bailer

2. Analysis of Protein-Protein Interactions Using LUMIER Assay

Sonja Blasche and Manfred Koegl

3. Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation

Stefan Becker and Jens von Einem

4. Discovery of Host-Viral Protein Complexes During Infection

Daniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea

5. Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi

Samantha J. Griffiths

6. High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine

Lars Dölken

7. Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape

Konstanze D. Scheffer, Ruth Popa-Wagner and Luise Florin

8. Computational Analysis of Virus-Host Interactomes

Caroline C. Friedel

9. Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins

Shuai Wang, Kezheng Wang, andChunfu Zheng

10. Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH)

Benedikt Kaufer

11. Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes

Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich

12. Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line

Diana Lieber and Susanne M. Bailer

13. Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs

Diana Lieber

14. Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry

Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger

15. High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time

Ruben Martinez, Andrew M. Burrage, Christopher M. Wiethoff, and Harald Wodrich

16. Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells

Martin Schauflinger,Clarissa Villinger, andPaul Walther

17. 3D Tissue Model for Herpes Simplex Virus-1 Infections

Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer

18. In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI)

Wali Hafezi and Verena Hoerr

19. Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry

Tina Schmidt and Martina Sester

20. Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells

Rebecca J. Aicheler and Richard J. Stanton

21. Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages

Zeguang Wu, Giada Frascaroli, and Thomas Mertens

22. Analysis of Cell Migration During HCMV Infection

Stefania Varani and Giada Frascaroli

23. Gene Targeting in Mice: A Review

Hicham Bouabe and Klaus Okkenhaug

24. A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells

Hicham Bouabe and Klaus Okkenhaug

25. Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells

Evgenia Scheibe, Stefan Lienenklaus, Tobias May, Vladimir Goncalves Magalhaes, Siegfried Weiss, and Melanie M. Brinkmann

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)