Vitrification in Assisted Reproduction: A User's Manual and Trouble-shooting Guide

Overview

After 20 years of the first publication on vitrification as an ice crystal-free cryopreservation method for embryos, there is a chronic need for a book that addresses this cryopreservation technique in detail, outlining those techniques that result in success, and providing recommended means of overcoming the problems typically encountered. The book will supply a historical overview of the principles and development of vitrification techniques in animals and humans; the various consumables utilized for the ...

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Overview

After 20 years of the first publication on vitrification as an ice crystal-free cryopreservation method for embryos, there is a chronic need for a book that addresses this cryopreservation technique in detail, outlining those techniques that result in success, and providing recommended means of overcoming the problems typically encountered. The book will supply a historical overview of the principles and development of vitrification techniques in animals and humans; the various consumables utilized for the vitrification of cells and tissue and suppliers thereof; and the vitrification and warming technique for oocytes, sperm, pronuclear stage embryos, cleavage stage embryos, blastocysts, ovarian tissue, and embryonic stem cells. Wherever applicable, the set-up including protocol, operation, maintenance, and trouble-shooting of the different protocols used for vitrifying various stages of cells and tissues will also be covered.

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Product Details

Meet the Author

Michael Tucker
Michael Tucker

Rob Cooper is a Test Lead on the Access team at Microsoft. He started at Microsoft as a support engineer in Charlotte, North Carolina in 1998 and joined the Access 2003 test team in Redmond in 2001. During the Access 2007 release, he led the security efforts across the test team and worked on several new features including disabled mode, database encryption, Office Trust Center, and sorting, grouping, and totals. Rob also led efforts around the Access object model and continues to provide direction around programmability and security in Access.

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Table of Contents

1a. Vitrification: an overview 1b. Basic biophysics and thermodynamics of vitrification 2. Disadvantages and benefits of vitrification 3. Development of vitrification solutions 4a. Vitrification in animal reproduction: vitrification of embryos using open pulled straws 4b. Vitrification in animal reproduction: vitrification of embryos using conventional straws with ethylene glycol-based solutions 5. Cryoprotectant-freevitrification of spermatozoa. 6. Potential developmental consequences of cryopreservation of mammalian oocytes and embryos 7a. Vitrification of oocytes: general considerations and the use of the Cryotop method 7b. Vitrification of oocytes: various procedures 7c. Vitrification of oocytes using gold grid and slush nitrogen 7d. Vitrifying and warming of oocytes using Cryotop 8. Vitrification of pronuclear embryos 9. Vitrification of day 2-3 human embryos 10a. Vitrification of human embryos in straws, hemi-straws, and high security vitrification straws 10b. Vitrification of blastocysts: using the Cryoloop 10c. Vitrification of blastocysts using the electron microscope grid 10d. Vitrifying and warming of human blastocysts using the Cryotop 11a Vitrification of ovarian tissue 11b. Vitrification of ovarian tissue: organ recovery systems 12. Vitrification of human embryonic stem cells.

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