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Voltammetric Methods in Brain Systems offers neuroscientists at all levels of expertise a combination of theory and practical how-to instructions for using in vivo and on-line neurotransmitter measurements in their experimental brain systems. Coverage includes the entire spectrum of applications for in situ, brain slices, transplant media, cell cultures, and single cells. Emphasis is on fast measurements that reveal release-reuptake information on transmitter systems. How-to information ranges from ample detail on the preparation of electrodes, to practical experimental details, to the interpretation of results.
Written by many of the world's leading experts-often those whose laboratories have spearheaded work in the area-Voltammetric Methods in Brain Systems provides insightful views on all the best approaches and experimental techniques, with sufficient introductory material and practical detail to satisfy both newcomers and experienced researchers.
The book contains black-and-white illustrations.
Carbon Electrode Surface Chemistry: Optimization of Bioanalytical Performance. Diffusion and Ion Shifts in the Brain Extracellular Microenvironment and Their Relevance for Voltammetric Measurements: The Brain Is Not A Beaker: In Vitro vs In Vivo Voltammetry. Fast Cyclic Voltammetry in Brain Slices. Rapid Chronocoulometric Measurements of Norepinephrine Overflow and Clearance in CNS Tissues. Monitoring Dopamine and Noradrenaline Release in Central and Peripheral Nervous Systems with Treated and Untreated Carbon-Fiber Electrodes. Regional Differences in Dopamine Release, Uptake, and Diffusion Measured by Fast-Scan Cyclic Voltammetry. The Measurement of Brain Ascorbate and Its Link with Excitatory Amino Acid Neurotransmission. Voltammetric and Amperometric Probes for Single-Cell Analysis. Measurement of the Time-Resolved Kinetics of Biogenic Amine Release and Transporter Activity by Rotating Disk Electrode Voltammetry In Vitro. Index.