YAC Libraries: A User's Guide

YAC Libraries: A User's Guide

by Stephanie L. Nelson, Bernard H. Brownstein
     
 

An indispensable guide to an emerging technology, this unique book introduces detailed methods for the construction, screening, and use of DNA sequences cloned into yeast artificial chromosome (YAC) vectors and the applications of YACs to gene hunting. Also covered are the reconstruction of large genes and the analysis of YACs by insertion back into mammalian cells

Overview

An indispensable guide to an emerging technology, this unique book introduces detailed methods for the construction, screening, and use of DNA sequences cloned into yeast artificial chromosome (YAC) vectors and the applications of YACs to gene hunting. Also covered are the reconstruction of large genes and the analysis of YACs by insertion back into mammalian cells—a method that may lead to the therapeutic use of YAC clones. The methods described are drawn from work on human DNA, but are universal to yeast artificial chromosomes used in other organisms. YAC Libraries is the third volume in the UWBC Biotechnical Resource Series with Dr. Richard Burgess, Director of the University of Wisconsin Biotechnology center, as Series Editor. The series is dedicated to providing practical guidance to researchers exploring new technologies and services.

Editorial Reviews

From the Publisher
"Cover[s] a wide range of topics and methodologies." —Journal of Chemical Education

Booknews
For researchers who have not used it before, introduces the methods for constructing, screening, and using DNA sequences cloned into yeast artificial chromosome vectors for hunting genes. Among the topics are vectors and host strains, localizing genes on metaphase chromosomes, and cloning human disease genes. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Product Details

ISBN-13:
9780716770145
Publisher:
Oxford University Press, USA
Publication date:
01/28/1993
Series:
University of Wisconsin Biotechnology Center Biotechnical Resource Series
Pages:
240
Product dimensions:
6.00(w) x 8.94(h) x 0.46(d)

Meet the Author

Baylor College of Medicine

Washington University School of Medicine

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