Yeast Gene Analysis / Edition 2

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Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. Yeast Gene Analysis, Second Edition, edited by two leading experts in the field, focuses on Saccharomyces cerevisiae and represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions.

Originally published shortly after completion of the yeast genome sequence, this new edition covers many of the major genome-wide strategies that have been developed subsequently, for example, microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. However, the basic methods of yeast molecular biology such as strain construction, immunological methods, generating conditional alleles of essential genes and PCR-mediated gene targeting have not been forgotten, and up-to-date procedures for these and many other standard approaches are also included. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.

Key Features: Aimed at both the novice and the experienced yeast researcher, Provides a combination of background information, experimental protocols and technical tips, Includes new chapters on strain construction, genome-wide two-hybrid approaches, use of microarrays fortranscript analysis, real-time analysis of chromosome behaviour, FRET, synthetic gene array technology, protein arrays, chemical genomics, yeast prions, RNA gene analysis, mitochondrial gene function analysis, phylogenetic footprinting, discovering human gene function and predicting yeast gene function.

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Editorial Reviews

From the Publisher
Praise for the Volume
"The book is far more than a cookbook: it seeks to place techniques and experimental strategies in some critical perspective, and expert investigators provide an overview of the state of the art in many areas... it is a compendium packed with information, insights, and discussions of potential pitfalls and limitations, all provided by experienced practitioners. The book successfully brings together a diffuse literature on sets of tools, technologies, and know-how in an accessible format and represents a sound investment for anyone interested in gene analysis."
"This is another excellent addition to the Methods in Microbiology Series... This is an outstanding resource for both investigators and graduate students working in the field of yeast gene function."
Praise for the Series
"An invaluable resource for the detailed analysis of cellular gene function and genome architecture."
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Product Details

  • ISBN-13: 9780123694782
  • Publisher: Elsevier Science
  • Publication date: 7/23/2007
  • Series: Methods in Microbiology Series
  • Edition number: 2
  • Pages: 730
  • Product dimensions: 6.90 (w) x 9.70 (h) x 1.40 (d)

Meet the Author

Alistair Brown has led an active research group in yeast molecular biology for over 15 years. Having started at Glasgow University, his group is now based at Aberdeen University, studying gene function and regulation in Saccharomyces cerevisiae and the control of morphogenesis in Candida albicans. The Aberdeen Candida Group is now one of the largest academic groups studying the molecular physiology of C. albicans.

Mick Tuite is a Professor of Molecular Biology in the Department of Biosciences at the University of Kent, UK He has published over 100 research papers and reviews in the area of yeast molecular biology and genetics and is a member of the editorial board of the journals Yeast and Molecular Microbiology.

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Table of Contents

Series Advisors     viii
Contributors     ix
Preface   I Stansfield   MJR Stark     xiii
Introduction to Functional Analysis in Yeast   SG Oliver     1
Yeast Genetics and Strain Construction   I Stansfield   MJR Stark     23
Yeast Transformation   D Truong   RD Gietz     45
A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting)   CI Maeder   P Maier   M Knop     55
Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles   MJR Stark     79
Yeast Hybrid Approaches   E Izumchenko   M Wolfson   EA Golemis   IG Serebriiskii     103
Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions   SV Rajagopala   B Titz   P Uetz     139
Reporter Genes and Their Uses in Studying Yeast Gene Expression   T von der Haar   LJ Josse   LJ Byrne     165
Transcript Analysis: A Microarray Approach   A Hayes   JI Castrillo   SG Oliver   A Brass$dLAH Zeef     189
GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts   Q Gao   TU Tanaka   X He     221
Immunological Methods   EH Hettema   KR Ayscough     241
Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP   TN Davis   EGD Muller     269
Identification, Characterization, and Phenotypic Analysis of Covalently Linked Cell Wall Proteins   FM Klis   P De Groot   S Brul     281
Yeast Protein Microarrays   J Ptacek   M Snyder     303
Smart Genetic Screens   M Breitenbach   JR Dickinson   P Laun     331
High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae   AHY Tong   C Boone     369
Chemical Genomic Tools for Understanding Gene Function and Drug Action   C Nislow   G Giaever     387
RNA Gene Analysis   C Saveanu   M Fromont-Racine   A Jacquier     415
Analysis of Gene Function of Mitochondria   S Duvezin-Caubet   AS Reichert   W Neupert     445
Yeast Prions and Their Analysis In Vivo   MF Tuite   LJ Byrne   L Josse   F Ness   N Koloteva-Levine   B Cox     491
Metabolic Control in the Eukaryotic Cell, a Systems Biology Perspective   JI Castrillo   SG Oliver     527
Phylogenetic Footprinting   PF Cliften     551
Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery   HR Waterham   RJA Wanders     577
Bioinformatic Prediction of Yeast Gene Function   I Lee   R Narayanaswamy   EM Marcotte     597
Yeast Genetic Strain and Plasmid Collections   K-D Entian   P Kotter     629
Yeast Gene Analysis: The Remaining Challenges   MJR Stark   I Stansfield     667
Index     685
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Customer Reviews

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  • Anonymous

    Posted February 19, 2014

    Hey dec. 6 2013

    Have you read it. Some people are really interested in this sort of thing. It could be a great book.

    I rate five stars becuase this person took time to make this book. Think of how long it must have taken.......

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  • Anonymous

    Posted December 6, 2013

    This has to be priced wrong wow

    I would never pay this much for one book wow

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