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Yeast Genetics: Fundamental and Applied Aspects

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Product Details

  • ISBN-13: 9781461254935
  • Publisher: Springer New York
  • Publication date: 7/31/2012
  • Series: Springer Series in Molecular and Cell Biology
  • Edition description: Softcover reprint of the original 1st ed. 1983
  • Edition number: 1
  • Pages: 533
  • Product dimensions: 6.14 (w) x 9.21 (h) x 1.14 (d)

Table of Contents

1. Genetic Control of Cell Proliferation.- Genes Involved in Cell Division.- Gene Products Involved in Cell Division.- Temporal Organization of Cell Cycle Events.- The Start Concept.- Conditions Necessary to Start the Cell Cycle.- Mating Hormones and Start.- The Spindle Pole Body and Start.- Developmental Alternative to Mitosis.- Saccharomyces cerevisiae V. Schizosaccharomyces pombe.- References.- 2. Genetic Control and Gene Expression During Meiosis and Sporulation in Saccharomyces cerevisiae.- Outline of Sporulation in Saccharomyces cerevisiae.- Special Genetic Techniques.- Gene Function and Gene Expression During Sporulation.- References.- 3. Meiotic Gene Conversion in Yeast: Molecular and Experimental Perspectives.- A Mechanistic Overview.- The Study of Gene Conversion.- Methods for Studying Gene Conversion.- Gene Conversion and Unselected Tetrads.- Mismatch Repair and Postmeiotic Segregation (pms).- Fidelity of Conversion.- Conversion of Deletions and Insertions.- Specific Molecular Mismatches in Conversion and pms.- The Effect of Adjacent Heterozygosity on pms Profiles.- Conversion-Associated Outside Marker Exchange.- Position of the Associated Exchange.- Conversion of Adjacent Genes.- Two-, Three-, and Four-Point Intragenic Crosses.- Intrachromosomal Gene Conversion and Recombinant DNA Strategies.- Conversion of Insertions and Intrachromosomal Gene Conversion.- References.- 4. Radiation-Sensitive Mutants and Repair in Yeast.- Radiation Biology of Yeast.- Radiation-Sensitive Mutants.- Double-Mutant Interactions and Epistasis Groups.- Phenotypic Characteristics of Repair-Defective Mutants.- Biochemical Studies on UV-Induced Damage and Repair.- X-ray-Sensitive Mutants.- The Role of RAD Genes in Meiosis.- References.- 5. Nucleic Acid Relatedness Among Yeasts.- Nucleic Acid Isolation and Purification.- DNA Base Composition.- DNA Relatedness.- Comparison of Relatedness from Nucleic Acid Studies with That Determined by Other Methodologies.- Molecular Taxonomy as an Aid to Genetic Research.- References.- 6. Genetic Approaches to the Study of Protease Function and Proteolysis in Saccharomyces cerevisiae.- Intracellular Proteases and Protease Inhibitors of S.cerevisiae.- Genetics of Known Proteases and Protease Inhibitors.- Proteolytic Processes and the Role of Known Proteases in These Processes.- Summary and Conclusions.- References.- 7. Genetic Control of Flocculation.- Methodology.- Origin of Strains.- Genetic Analysis.- Phenotypic Variations.- Cell Wall Differences.- Stability of Flocculence.- Industrial Applications.- References.- 8. Protein and Lipid Composition of the Yeast Plasma Membrane.- Cytological Purity of Membrane Preparations.- A Vesicle Free Cell Wall Preparation.- Surface Labeling of Intact Yeast Cells.- One-Dimensional SDS-PAGE Surface Label Experiments.- Two-Dimensional SDS-PAGE.- Cell Wall Polypeptides.- Comparative Lipid Analyses.- Concluding Remarks.- References.- 9. Protoplast Fusion in Yeasts.- Protoplast Isolation, Fusion, and Reversion.- The Consequences of Protoplast Fusion.- Discussion.- References.- 10. Genetic and Functional Aspects of Yeast Mihondria.- Introduction: The Petite Mutant.- Recombination in Mihondria.- Antimihondrial Activity of Drugs.- Mihondria and Cellular Processes.- Conclusions.- References.- 11. Molecular Genetic Aspects of Yeast Mihondria.- General Introduction.- Mihondrial DNA.- The Diversity of Mihondrial DNAs.- Yeast Mihondrial DNA.- Mihondrial Transcription.- Mihondrial Protein Synthesis.- The Petite Mutation and mtDNA Replication.- Genetic Interactions Between Nucleus and Mihondrion.- Evolutionary Aspects.- Conclusions and Prospects.- References.- Note Added in Proof.- 12. ScV “Killer” Viruses in Yeast.- Viral Particles.- Viral Genomes.- Viral Maintenance.- Other Cytoplasmic Elements.- Conclusions.- References.- 13. Approaches to the Genetic Analysis and Breeding of Brewer’s Yeast.- Genetic Characterization of Brewing Strains.- Breeding of Brewing Strains.- References.- 14. Genetic Improvement of Wine Yeast.- Origin and Classification of Wine Yeasts.- Life Cycle and Ploidy Level.- Wine Yeast Improvement.- Genetics and Examples of Strain Improvement.- Strain Maintenance.- Concluding Remarks.- References.- 15. Aspects of the Biochemistry and Genetics of Sugar and Carbohydrate Uptake by Yeasts.- D-Galactose.- Maltose and Maltotriose.- Pentoses.- Extracellular Enzymes.- Sucrose.- Melibiose.- Dextrin and Starch.- Mihondrial Control of Sugar Utilization.- References.- Author Index.- Species Index.

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