Therapeutic Peptides: Methods and Protocols

Therapeutic Peptides: Methods and Protocols

by Andrew E. Nixon
ISBN-10:
1493957120
ISBN-13:
9781493957125
Pub. Date:
08/23/2016
Publisher:
Springer-Verlag New York, LLC
ISBN-10:
1493957120
ISBN-13:
9781493957125
Pub. Date:
08/23/2016
Publisher:
Springer-Verlag New York, LLC
Therapeutic Peptides: Methods and Protocols

Therapeutic Peptides: Methods and Protocols

by Andrew E. Nixon
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Overview

Therapeutic Peptides: Methods and Prools features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Practical and dependable, Therapeutic Peptides: Methods and Prools is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.


Product Details

ISBN-13: 9781493957125
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1088
Edition description: Softcover reprint of the original 1st ed. 2014
Pages: 279
Product dimensions: 7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

1. Design of Phage-Displayed Cystine-Stabilized Mini-Protein Libraries for Proteinaceous Binder Engineering

            Hung-Ju Chang and An-Suei Yang

2. Construction of a Filamentous Phage Display Peptide Library

            Annette Fagerlund, Astrid Hilde Myrset, and Mari Ann Kulseth

3. Engineering Bio-Active Peptide-Based Therapeutic Molecules

            Jong Sang Ryu, A. Yeon Cho, Sang Won Seo, and Hosung Min

4. T7 Lytic Phage-Displayed Peptide Libraries: Construction and Diversity Characterization

            Lauren R.H. Krumpe and Toshiyuki Mori

5. Affinity Selection Using Filamentous Phage Display

            Mari Ann Kulseth, Annette Fagerlund, and Astrid Hilde Myrset

6. Bioprospecting Open Reading Frames for Peptide Effectors

            Ling Xiong and Charles Scott

7. Identification of Ideal Peptides for Heterovalent Ligands

            Ajay Shrivastava, Matthew von Wronski, Michael F. Tweedle, and Adrian D. Nunn

8. Substrate Phage Display for Protease Substrate Sequence Characterization: Bovine Factor Xa as a Model System

            Hung-Ju Hsu and An-Suei Yang

9. Engineering Peptide Therapeutics Using MIMETIBODY™ Technology

            Kristen Picha, Chichi Huang, Peter Bugelski, and Karyn O’Neil

10. Evaluation of Peptides as Protease Inhibitors and Stimulators

            Hannu Koistinen, Can Hekim, Ping Wu, Ale Närvänen, and Ulf-Håkan Stenman

11. Assessment of Antimicrobial (Host Defense) Peptides as Anti-Cancer Agents

            Susan Douglas, David Hoskin, and Ashley Hilchie

12. Peptide Labelling Strategies for Imaging Agents

            Tuulia Huhtala, Janne Weisell, Jussi Rytkönen, and Ale Närvänen

13. Peptide Optimization and Conjugation Strategies in the Development of Molecularly Targeted Magnetic Resonance Imaging Contrast Agents

            Andrew F. Kolodziej, Zhaoda Zhang, Kirsten Overoye-Chan, Vincent Jacques, and Peter Caravan

14. Evaluation of Prenylated Peptides for Use in Cellular Imaging and Biochemical Analysis

            Joshua D. Ochocki, Urule Igbavboa, W. Gibson Wood, Elizabeth V. Wattenberg, and Mark D. Distefano

15. Ultraviolet Absorption Spectroscopy of Peptides

            Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh

16. Fluorescence Spectroscopy of Peptides

            Mangala R. Liyanage, Kunal Bakshi, David B. Volkin, and C. Russell Middaugh

17. Circular Dichroism of Peptides

            Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh

18. Fourier Transform Infrared Spectroscopy of Peptides

            Kunal Bakshi, Mangala R. Liyanage, David B. Volkin, and C. Russell Middaugh

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