Discover the experimental and theoretical developments inoptical single-molecule spectroscopy that are changing the ways wethink about molecules and atoms
The Advances in Chemical Physics series provides thechemical physics field with a forum for critical, authoritativeevaluations of advances in every area of the discipline. Thislatest volume explores the advent of optical single-moleculespectroscopy, and how atomic force microscopy has empowered novelexperiments on individual biomolecules, opening up new frontiers inmolecular and cell biology and leading to new theoreticalapproaches and insights. Organized into two parts—oneexperimental, the other theoretical—this volume exploresadvances across the field of single-molecule biophysics, presentingnew perspectives on the theoretical properties of atoms andmolecules. Single-molecule experiments have provided freshperspectives on questions such as how proteins fold to specificconformations from highly heterogeneous structures, how signaltransductions take place on the molecular level, and how proteinsbehave in membranes and living cells.This volume is designed tofurther contribute to the rapid development of single-moleculebiophysics research.
Filled with cutting-edge research reported in a cohesive mannernot found elsewhere in the literature, each volume of theAdvances in Chemical Physics series serves as the perfectsupplement to any advanced graduate class devoted to the study ofchemical physics.
About the Author
Volume EditorsTamiki Komatsuzaki is a Professor in the Molecule and LifeNonlinear Sciences Laboratory, Research Institute for ElectronicScience at Hokkaido University in Japan. His research interestsinclude origin of selectivity and stochasticity of reactions,single-molecule biophysics, as well as protein landscape andcomplexity in kinetics and dynamics.
Masaru Kawakami is an Associate Professor at the JapanAdvanced Institute of Science and Technology (JAIST) School ofMaterial Science. He specializes in biophysics, single-moleculemeasurement, protein folding, and structural biology, and hisresearch focuses on single-molecule dynamics of biomolecules.
Satoshi Takahashi is a Professor at the Institute ofMultidisciplinary Research for Advanced Materials, TohokuUniversity, Japan. His research focuses on protein folding andfunctional dynamics.
Haw Yang is an Associate Professor in the Department ofChemistry at Princeton University. His current research focuses onhigh-resolution quantitative single-molecule protein dynamics andreal-time 3D single-particle tracking spectroscopy.
Robert J. Silbey is the Class of 1942 Professor ofChemistry at MIT. His research involves theoretical studies ofsingle-molecule spectroscopy at low temperatures, energy andelectron transfer and relaxation in molecular aggregates, theoptical and electronic properties of conjugated oligomers andpolymers, and the transport of charge in organic systems.
Series EditorsStuart A. Rice received his master's and doctorate from HarvardUniversity and was a junior fellow at Harvard for two years beforejoining the faculty of the University of Chicago in 1957, where heis currently the Frank P. Hixon Distinguished Service ProfessorEmeritus.
Aaron R. Dinner received his bachelor's degree anddoctorate from Harvard University, after which he conductedpostdoctoral research at the University of Oxford and theUniversity of California, Berkeley. He joined the faculty at theUniversity of Chicago in 2003.
Table of Contents
Part One Developments on Single-Molecule Experiments
Staring at a Protein: Ensemble and Single-MoleculeInvestigations on Protein-Folding Dynamics 3By Satoshi Takahashi and Kiyoto Kamagata
Single-Molecule FRET of Protein-Folding Dynamics 23By Daniel Nettels and Benjamin Schuler
Quantitative Analysis of Single-Molecule FRET Signals and itsApplication to Telomere DNA 49By Kenji Okamoto and Masahide Terazima
Force to Unbind Ligand–Receptor Complexes and the InternalRigidity of Globular Proteins Probed by Single-Molecule ForceSpectroscopy 71By Atsushi Ikai, Rehana Afrin, and Hiroshi Sekiguchi
Recent Advances in Single-Molecule Biophysics with the Use ofAtomic Force Microscopy 89By Masaru Kawakami and Yukinori Taniguchi
Dynamical Single-Molecule Observations of Membrane Protein UsingHigh-Energy Probes 133By Yuji C. Sasaki
Single-Molecular Gating Dynamics for the KcsA Potassium Channel147By Shigetoshi Oiki, Hirofumi Shimizu, Masayuki Iwamoto, andTakashi Konno
Static and Dynamic Disorder in IN VITRO ReconstitutedReceptor–Adaptor Interaction 195By Hiroaki Takagi, Miki Morimatsu, and Yasushi Sako
Part Two Developments on Single-Molecule Theories andAnalyses
Change-Point Localization and Wavelet Spectral Analysis ofSingle-Molecule Time Series 219By Haw Yang
Theory of Single-Molecule FRET Efficiency Histograms 245By Irina V. Gopich and Attila Szabo
Multidimensional Energy Landscapes in Single-Molecule Biophysics299By Akinori Baba and Tamiki Komatsuzaki
Generalized Michaelis–Menten Equation for ConformationModulated Monomeric Enzymes 329By Jianlan Wu and Jianshu Cao
Making it Possible: Constructing a Reliable Mechanism from aFinite Trajectory 367By Ophir Flomenbom
Free Energy Landscapes of Proteins: Insights from MechanicalProbes 395By Zu Thur Yew, Peter D. Olmsted, and Emanuele Paci
Mechanochemical Coupling Revealed by the Fluctuation Analysis ofDifferent Biomolecular Motors 419By Hiroaki Takagi and Masatoshi Nishikawa
Author Index 437
Subject Index 467