ISBN-10:
0824785681
ISBN-13:
9780824785680
Pub. Date:
09/24/1991
Publisher:
Taylor & Francis
Advances in Chromatography / Edition 1

Advances in Chromatography / Edition 1

by J. Calvin Giddings
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Product Details

ISBN-13: 9780824785680
Publisher: Taylor & Francis
Publication date: 09/24/1991
Series: Advances in Chromatography Series , #31
Pages: 416
Product dimensions: 8.50(w) x 11.00(h) x 1.06(d)

Table of Contents

Contributorsiii
Contents of Other Volumesix
Chapter 1Roles of High-Performance Liquid Chromatography in Nuclear Medicine1
I.Introduction2
II.Characteristics of Radiopharmaceuticals3
III.Instrumentation5
IV.Preparation and Purification6
V.Analysis of [superscript 99m]Tc-Labeled Pharmaceuticals28
VI.Conclusion31
Acknowledgments32
References32
Chapter 2Calibration of Separation Systems in Gel Permeation Chromatography for Polymer Characterization37
I.Introduction38
II.Calibration Using Standards with Narrow Distributions39
III.Calibration Using Standards with Broad Distribution46
IV.Universal Calibration52
V.Special Procedures of Calibration71
VI.Influence of Operational Variables on Results of Calibration72
VII.Conclusions78
Acknowledgments79
AppendixApplications of Universal Calibration Principles in Synthetic Polymer Characterization80
References80
Chapter 3Isomer-Specific Assay of 2,4-D Herbicide Products By HPLC: Regulatory Methodology91
I.Introduction91
II.Ion-Exchange Chromatography Versus Reverse-Phase Chromatography93
III.Method Criteria94
IV.Impurities in 2,4-D94
V.Separation of Impurities94
VI.Alternative Columns100
VII.Column Stability107
VIII.Precision and Linearity107
IX.Mobile Phase Modifier108
X.Conclusions108
References108
Chapter 4Hydrophobic Interaction Chromatography111
I.Introduction112
II.Definition of Hydrophobic Interaction and the Structure of Water112
III.Changes in the Water Structure upon Solubilization of a Nonpolar Compound in Water113
IV.Chemical Nature of the Hydrophobic Interaction113
V.Charged Amphiphilic Adsorbents115
VI.Neutral Amphiphilic Adsorbents116
VII.Multipoint Interaction117
VIII.Establishment of Appropriate Experimental Conditions for a Run118
IX.Applications119
Acknowledgments121
References121
Chapter 5Liquid Chromatography with Programmed Composition of the Mobile Phase125
I.Introduction126
II.Retention Characteristics in Isocratic-Elution Chromatography and in Chromatography with Programmed Composition of the Mobile Phase128
III.Practical Aspects of Chromatography with Programmed Composition of the Mobile Phase235
IV.Conclusions248
V.Summary248
Appendix AList of Symbols Used249
References253
Chapter 6Chromatographic Separation of Aldosterone and Its Metabolites261
I.Introduction262
II.Metabolism and Mechanism of Action of Aldosterone263
III.Separation and Column Chromatography of Reduced and Polar Aldosterone Metabolites264
IV.High-Pressure Liquid Chromatography of Reduced and Polar Aldosterone Metabolites268
V.Improved Separations of Aldosterone Metabolites Using High-Pressure Liquid Chromatography273
VI.High-Pressure Liquid Chromatography of Aldosterone Metabolites Synthesized in Vitro275
VII.Aldosterone Metabolites in Male Rat Urine281
VIII.Conclusions282
Acknowledgments283
References283
Author Index287
Subject Index307

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