This reference presents the most recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds. The authors offer strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate developments in controlled drug delivery and targeting. The book contains current applications and procedures to identify binding constants between drugs, biological structures, proteins, nucleic acids, and plasmids, increase drug bioavailability, enhance the permeability, solubility, strength, and function of drug formulations, examine protein-protein exchanges and reactions, and calculate pKa values.
Table of ContentsPrinciples and theory: principles of capillary electrophoresis; theory of affinity electrophoresis. Application of affinity capillary electrophoresis in pharmaceutics: determination of physicochemical parameters; affinity of drugs to excipients; affinity of drugs to pharmaceutical vehicle systems - micelles. Application of affinity capillary electrophoresis in biopharmaceutics: affinity capillary electrophoresis - DNA interactions with peptides, proteins and modified DNAs; characterization of polysaccheride interactions; characterization of immunoreactions. New combination techniques and conclusions: hyphenation of affinity capillary electrophoresis with mass spectrometry.