ISBN-10:
1617372439
ISBN-13:
9781617372438
Pub. Date:
11/09/2010
Publisher:
Springer-Verlag New York, LLC
Allergy Methods and Protocols / Edition 1

Allergy Methods and Protocols / Edition 1

by Meinir G. Jones, Penny Lympany

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Product Details

ISBN-13: 9781617372438
Publisher: Springer-Verlag New York, LLC
Publication date: 11/09/2010
Series: Methods in Molecular Medicine , #138
Edition description: Softcover reprint of hardcover 1st ed. 2008
Pages: 352
Product dimensions: 5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

List of chapters

1. Understanding the molecular mechanisms of allergy - Meinir Jones
2. T cell - primary culture from peripheral blood - Monika
Raulf-Heimsoth
3. Production of T cell lines - Helga Kahlert
4. Production of human T cell clones - Adrienne Verhoef
5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler and
Tuomas Virtanen
6. Determining MHC restriction of T cell response - Mark Larche
7. Short term culture of CD8 cells and intracellular cytokine staining
- Beejal Vyas and Alistair Noble
8. Isolation, flow cytometric analysis and suppression assay of
CD4+CD25+ T regulatory cells - Hayley Jeal
9. Monocyte-derived dendritic cells as antigen presenting cells in T
cell proliferation and cytokine production - Sun-sang J Sung
10. Ultrasensitive ELISA for measurement of human cytokine responses in primary culture - William P. Stefura, J. Darren Campbell, Renée
Douville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, and
Kent T. HayGlass
11. Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses - Monique Stinson, Renee Douville,
Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons,
Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass
12. Standardisation of allergen extracts - Jørgen Nedergaard Larsen and
Sten Dreborg
13. Immuno-electrophoresis for the characterisation of allergen extracts - Gitte Nordskov Hansen and Jørgen Nedergaard Larsen
14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol
15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell
16. Purification of antibodies - Per H Larsson and Anne Renström
17. Collection of air samples to quantify exposure to airborne allergens - Susan Gordon
18. Assay of air sample eluates - Anne Renström and Susan Gordon
19. The halogen assay - a new technique for measuring airborne allergen
- Euan Tovey,Sandra De Lucca, Leanne Poulos and Tim O'Meara
20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones
21. The facilitated-antigen binding (FAB) assay - a prool to measure allergen-specific inhibitory activity - James N Francis
22. Microscopic identification and purity determination of pollen grains - Magdalena Rahl
23. Biopanning for the characterisation of allergen mimotopes -
Isabella Schöll and Erika Jensen-Jarolim
24. Identification of mast cells and mast cell subpopulations - Mark
Buckley and Andrew F Walls
25. Purification and characterisation of mast cell tryptase and chymase
- Alan R McEuen and Andrew F Walls
26. Experimental activation of mast cells and their pharmacological modulation - Shaoheng He and Andrew F Walls
27. In situ hybridisation - Kayhan T. Nouri-Aria

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