Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols

Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols

by Erik D. Roberson (Editor)


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Product Details

ISBN-13: 9781607617433
Publisher: Springer-Verlag New York, LLC
Publication date: 10/25/2010
Series: Methods in Molecular Biology , #670
Edition description: 2011
Pages: 277
Product dimensions: 7.20(w) x 10.20(h) x 0.90(d)

Table of Contents

1. Contemporary Approaches to Alzheimer’s Disease and Frontotemporal Dementia
Erik D. Roberson

Part I: Working with AD- and FTD-Related Molecules

2. Preparing Synthetic A in Different Aggregation States
W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, and Mary Jo LaDu

3. Isolation of Low-n Amyloid β-Protein Oligomers from Cultured Cells, CSF, and Brain
Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, and Dominic M. Walsh

4. Detecting A*56 Oligomers in Brain Tissues
Mathew A. Sherman and Sylvain E. Lesné

5. Assessing A Aggregation State by Atomic Force Microscopy
Justin Legleiter

6. Measuring APP Carboxy-Terminal Fragments
Luke A. Esposito

7. Detection of APP Intracellular Domain (AICD) in Brain Tissue
Sanjay W. Pimplikar and Anupama Suryanarayana

8. Cell-Based Assays for Regulators of Tau Biology
Umesh K. Jinwal and Chad A. Dickey

9. Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In situ
Wanjoo Chun, Geoffrey S. Waldo, and Gail V.W. Johnson

10. Apolipoprotein E Expression and Purification
Yvonne Newhouse and Karl H. Weisgraber

Part II: Model Systems and Outcome Measures

11. Aβ Toxicity in Primary Cultured Neurons
Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, and Sara Kleinschmidt

12. Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors
Binggui Sun and Li Gan

13. Selecting a Mouse Model of Alzheimer’s Disease
Jeannie Chin

14. Monitoring Spatial Learning and Memory in Alzheimer’s Disease Mouse Models Using the Morris Water Maze
Kimberly Scearce-Levie

15. Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain
Jorge J. Palop, Erik D. Roberson, and Inma Cobos

16. Real-Time Visualization of Axonal Transport in Neurons
Yasuko Osakada and Bianxiao Cui

17. Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer’s Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling
Jorge J. Palop, Lennart Mucke, and Erik D. Roberson

18. Epigenetic Changes in the Brain: Measuring Global Histone Modifications
Gavin Rumbaugh and Courtney A. Miller

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