Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols

by Erik D. Roberson (Editor)

Overview

Alzheimer’s disease and frontotemporal dementia, two of the most prevalent neurodegenerative diseases, are dreaded disorders that attack the neural networks underlying memory and personality, systems that make us who we are. Major breakthroughs in recent years have spurred new research approaches to these conditions. In Alzheimer's Disease and Frontotemporal Dementia: Methods and Prools, expert investigators bring together the many divergent areas of expertise used to study these diseases, including behavior, electrophysiology, confocal microscopy, and hardcore biochemistry. Beginning with an overview of the two diseases and contemporary research on them, the book continues with specialized prools for working with amyloid-β peptide, tau, and apolipoprotein E as well as experimental systems for studying AD and FTD, including cell and animal models, and outcome measures that can be used to assess neuronal function in these systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Alzheimer's Disease and Frontotemporal Dementia: Methods and Prools serves as an ideal guide for all scientists pursuing the cause and cure of these widespread and nightmarishly debilitating disorders.

Product Details

ISBN-13:	9781607617433
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/25/2010
Series:	Methods in Molecular Biology , #670
Edition description:	2011
Pages:	277
Product dimensions:	7.20(w) x 10.20(h) x 0.90(d)

Table of Contents

Contemporary Approaches to Alzheimer’s Disease and Frontotemporal Dementia.- Preparing Synthetic A in Different Aggregation States.- Isolation of Low-n Amyloid β-Protein Oligomers from Cultured Cells, CSF, and Brain.- Detecting A*56 Oligomers in Brain Tissues.- Assessing A Aggregation State by Atomic Force Microscopy.- Measuring APP Carboxy-Terminal Fragments.- Detection of APP Intracellular Domain (AICD) in Brain Tissue.- Cell-Based Assays for Regulators of Tau Biology.- Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In situ.- Apolipoprotein E Expression and Purification.- Aβ Toxicity in Primary Cultured Neurons.- Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors.- Selecting a Mouse Model of Alzheimer’s Disease.- Monitoring Spatial Learning and Memory in Alzheimer’s Disease Mouse Models Using the Morris Water Maze.- Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain.- Real-Time Visualization of Axonal Transport in Neurons.- Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer’s Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling.- Epigenetic Changes in the Brain: Measuring Global Histone Modifications.

Editorial Reviews

From the reviews:

“This book brings together research authorities in the field of Alzheimer’s disease (AD) and frontotemporal dementia (FTD) to share their laboratory methods and prools for molecular and model systems and outcome research in the field of neurodegenerative biomedical research. … The audience includes all laboratory scientists interested in molecular and neuroscience research that seeks a cause and cure for these two devastating disorders. … The book is thoughtfully written and will have the greatest appeal for those with a familiarity with laboratory research methods.” (Charlene Hoffman Snyder, Doody’s Review Service, February, 2011)

“This is a collection of relatively modern methodologies, focused primarily on the study of amyloid-β processing … . Research technicians will find the ‘notes’ sections particularly valuable … . provides a nice reference for anyone interested in studying putative models of Alzheimer’s disease. … Overall, the book is well organized, well written, appropriately succinct, and exceedingly useful in those areas that are addressed, and provides a valuable reference to the basic scientist studying Alzheimer’s disease.” (Rudy J. Castellani, Journal of Alzheimer’s Disease, Vol. 25, 2011)

From the Publisher