Animal Cell Culture / Edition 1 available in Hardcover
- Pub. Date:
- Springer-Verlag New York, LLC
Animal Cell Culture, the latest volume in Humana's highly successful Methods in Molecular Biology series, provides detailed practical techniques for the culture of a broad spectrum of basic cell cell types. Chapters offer hands-on methods for creating mammalian fibroblastic cell cultures and maintaining culture conditions for epithelial, neuronal, and hematopoietic cells among others. Attention is given to the diversity of culture media and extracellular matrices needed to maintain the differentiated functions of the cultured cells.
The book's special strength lies in its descriptions of culture techniques for both living and fixed cells. Chapters cover techniques such as: • cinematographic analysis • in situ mRNA hybridization • immunofluorescence • immunoelectron microscopy • somatic cell hybridization • DNA transformation • insect cell culture • creation of hybridoma cell lines • monoclonal antibody techniques • new, specialized methodologies. A useful appendix lists the most commonly used culture media.
Comprehensive in scope and coverage, and thoroughly up-to-date, Pollard and Walker's unique handbook on ANIMAL CELL CULTURE is an indispensable resource for both the novice and the seasoned expert.
Table of Contents
Basic Cell Culture.- Establishment, Maintenance, and Cloning of Human Primary Cell Strains.- Aging of Cultured Human Skin Fibroblasts.- Separation and Maintenance of Primary T and B Lymphocytes.- Establishment of Lymphoblastoid Cell Lines.- Scale-Up of Suspension and Anchorage-Dependent Animal Cells.- Mycoplasma Detection.- Short-Term Chorionic Villi and Amniotic Fluid Cultures.- Keratinocyte Culture.- Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines.- Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells.- Embryonic Rodent Brain Cells in Culture.- Human Thyroid Epithelial Cells.- Preparation of Isolated Rat Liver Hepaytes.- Primary Cultures of Rat Hepaytes.- Preparation of Isolated Periportal or Perivenous Hepaytes from Rat Liver.- Primary Kidney Cells.- Adipocytes.- Tissue Culture of Skeletal Muscle.- Derivation and Maintenance of Embryonic Stem Cell Cultures.- Defining Hormone and Matrix Requirements for Differentiated Epithelia.- Culturing Primitive Hemopoietic Cells.- High Proliferative Potential Colony Forming Cells.- Murine Bone Marrow-Derived Macrophages.- Long-Term B Lymphoid Cultures from Murine Bone Marrow.- CFU-C in Agar.- Human Long-Term Bone Marrow Culture.- In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells.- Flow Sorting for Isolating CFU-E.- Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors.- Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries.- Cytogenetic Techniques for Human Leukemias.- Time-Lapse Cinemicroscopy.- Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells.- Double Indirect-Immunofluorescent Labeling of Cultured Cells.- Gene Mapping to Chromosomes by Hybridization in Situ.- In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears.- Immunogold Labeling for Electron Microscopy.- Human Chromosome Analysis and Sorting.- Flow Cytometry.- Fluorescence-Activated Cell Sorting According to Receptor Density.- Transfection and Transformation of Human Thyroid Epithelial Cells.- Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector.- Chloramphenicol Acetyltransferase as a Reporter in Mammalian Gene Transfer.- Immunizing Schedules for Hybridoma Production.- Fusion Prool for the Production of Mouse Hybridomas.- Cloning of Hybridomas.- Enzyme-Linked Immunosorbent Assay for Screening of Antibodies in Hybridoma Supernatants.- Cryopreservation of Hybridomas.- Isotype Determination of Monoclonal Antibodies by Immunodiffusion.- Isoelectric Focusing and Immunofixation of Monoclonal Antibodies.- Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas.- Purification of Murine Monoclonal Antibodies.- Rat x Rat Hybridomas.- Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides.