Antibody Methods and Protocols

Antibody Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2012)

$161.10 $179.00 Save 10% Current price is $161.1, Original price is $179. You Save 10%.
View All Available Formats & Editions
Choose Expedited Shipping at checkout for guaranteed delivery by Monday, July 22

Overview

The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

Product Details

ISBN-13: 9781493959303
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #901
Edition description: Softcover reprint of the original 1st ed. 2012
Pages: 325
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1. Antigen Presentation for the Generation of Binding Molecules

Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang

2. Recombinant Antibodies and In Vitro Selection Technologies

C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu

3. Phage Display

Konstantin Petropoulos

4. Generation of Human Fab Libraries for Phage Display

Christoph Rader

5. Selection of Human Fab Libraries by Phage Display

Christoph Rader

6. Ribosome Display

George Thom and Maria Groves

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

Chonghui Zhang

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

E-Chiang Lee and Michael Owen

9. Production of Human or Humanized Antibodies in Mice

Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schräml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Konstantin Petropoulos

4. Generation of Human Fab Libraries for Phage Display

Christoph Rader

5. Selection of Human Fab Libraries by Phage Display

Christoph Rader

6. Ribosome Display

George Thom and Maria Groves

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

Chonghui Zhang

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

E-Chiang Lee and Michael Owen

9. Production of Human or Humanized Antibodies in Mice

Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schräml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schräml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schräml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Michael Schräml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Michael Schräml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Simona Jevševar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogné

Virginie Pascal, Brice Laffleur, and Michel Cogné

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews