Automation, edited by two of the leading experts in the field, presents the very latest experimental techniques explained in detail. The book is structured for ease of reference, and is essential reading for all researchers working in genomics, biochemistry, molecular and cell biology, genetics, microbiology and robotics as well as those in the parmaceutical/biotechnology industry.
Key features and contents:
• Describes automated procedures used in microbiology and molecular biology
• Includes developments in robotics and vision systems
• Features automation in library picking, presentation and analysis
• Discusses paralogous duplications in microbial genomes
• Covers deciphering genomes through automated large-scale sequencing
• Describes and stresses the need for functional analyses
• Internationally acclaimed contributors, including Professor Leroy Hood
Automation is the major future trend in all biomedical research areas requiring high-throughput analyses. Techniques and assays that were once repetitive, tedious and time consuming, can be performed robotically, liberating the time of laboratory researchers for more interesting work. A multi-author book, this volume has succeeded in bringing together researchers at the forefront of clone library construction, genome analysis, sequencing, computational data evaluation and functional analyses to provide insight into this "new age" of research based on genomic and chemical screening.
Established for almost 30 years, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalised, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.
About the Author
Table of ContentsL. Hood, Foreword.
U. Pettersson, Introduction.
A. Fife and D.W.M. Crook, Automation in Clinical Microbiology.
A.J.McCollum, Vision Systems for Automated Colony and Plaque Picking.
D.R. Bancroft, E. Maier and H. Lehrach, Library Picking, Presentation and Analysis.
G. Kauer and H. Blöcker, The PREPSEQ Robot: An Integrated Environment for Fully Automated and Unattended Plasmid Preparations and Sequencing Reactions.
A.N. Hale, Building Realistic Automated Production Lines for Genetic Analysis.
A.N. Hale, Examples of Automated Genetic Analysis Developments.
L. Rowen, S. Lasky and L. Hood, Deciphering Genomes Through Automated Large-scale Sequencing.
N.C. Hauser, M. Scheideler, S. Matysiak, M. Vingron and J.D. Hoheisel, DNA Arrays for Transcriptional Profiling.
K.-J. Reiger, G. Orlowska, A. Kaniak, J.-Y. Coppee, G. Alijinovic and P.P. Slonimski, Large-scale Phenotypic Analysis in Microtitre Plates of Mutants with Deleted Open Reading Frames From Yeast Chromosome III: Key-step Between Genomic Sequencing And Protein Function.
J.J. Codani, J.P. Comet, J.C. Aude, E. Glémet, A. Wozniak, J.L. Risler, A. Hénaut and P.P. Slonimski, Automatic Analysis of Large-scale Pairwise Alignments of Protein Sequences.
M.Y. Galperin and D. Frishman, Towards Automated Prediction of Protein Function from Microbial Genomic Sequences.