Pub. Date:
Springer-Verlag New York, LLC
Bacterial Pathogenesis: Methods and Protocols / Edition 1

Bacterial Pathogenesis: Methods and Protocols / Edition 1

by Frank DeLeo, Michael Otto


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Bacterial Pathogenesis: Methods and Protocols / Edition 1

Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.

Product Details

ISBN-13: 9781617377518
Publisher: Springer-Verlag New York, LLC
Publication date: 12/09/2010
Series: Methods in Molecular Biology , #431
Edition description: Softcover reprint of hardcover 1st ed. 2008
Pages: 320
Product dimensions: 6.10(w) x 9.25(h) x 0.24(d)

Table of Contents

Part I. Mechanisms of Bacterial Pathogenesis 1 Isolation and Characterization of Lipopolysaccharides Michael A. Apicella 2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes Michelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee 3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus Susanne Engelmann and Michael Hecker 4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution Caitriona M. Guinane and J. Ross Fitzgerald 5 Detection and Inhibition of Bacterial Cell-Cell Communication Scott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg 6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii James M. Battisti, Sandra J. Raffel, and Tom G. Schwan 7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi Philip E. Stewart and Patricia A. Rosa 8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) – A Molecular and Biochemical Approach Cuong Vuong and Michael Otto Part II. Host-Pathogen Interaction 9 Analysis of Staphylococcus aureus Gene Expression during PMN Phagocytosis Jovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo 10 Examining the Vector-Host-Pathogen Interface with Quantitative Molecular Tools Jason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch 11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages Jean Celli 12 Rate and Extent of Helicobacter pylori Phagocytosis Lee-Ann H. Allen 13 Culture, Isolation and Labeling of Anaplasma Phagocytophilum for Subsequent Infection ofHuman Neutrophils Dori L. Borjesson 14 Ultrastructural Analysis of Bacteria-Host Cell Interactions David W. Dorward 15 Infection of Human Monocyte-derived Macrophages with Coxiella burnetii Jeffrey G. Shannon and Robert A. Heinzen 16 Infection of Epithelial Cells with Salmonella enterica Olivia Steele-Mortimer 17 Determining the Cellular Targets of ROS in Borrelia burgdorferi Julie A. Boylan and Frank C. Gherardini Part III. Animal Models of Bacterial Infection 18 Bioluminescent Imaging of Bacterial Biofilm Infections in vivo Jagath L. Kadurugamuwa and Kevin P. Francis 19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans (Cotton Rat S. aureus Nasal Colonization Model) John Kokai-Kun 20 A Non-Human Primate Model of Acute Group A Streptococcus Pharyngitis Paul Sumby, Anne H. Tart, and James M. Musser Part IV. Identification of Therapeutic Targets and Typing Methods 21 Target-based Antimicrobial Drug Discovery Lefa E. Alksne and Paul M. Dunman 22 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa Typing Barun Mathema, Jose Mediavilla, and Barry N. Kreiswirth

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