Pub. Date:
Springer-Verlag New York, LLC
Basic Cell Culture Protocols / Edition 3

Basic Cell Culture Protocols / Edition 3

by Cheryl D. Helgason, Cindy L. Miller


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Basic Cell Culture Protocols / Edition 3

In introducing two dozen contributions, Helgason (cancer endocrinology, British Columbia Cancer Agency, Canada) and Miller (StemCell Technologies, Inc., Vancouver, BC) note that this laboratory manual is not intended to educate tissue cell culturists on any specific cell type or organ system. Rather, they offer step-by- step instructions on methodologies and equipment needed to enable biomedical researchers to isolate, culture, characterize, and maintain major primary cell types including stem cells. New techniques covered include leukocyte rolling and scalable production of ES-derived cells. The companion CD contains the text in an eBook/PDA version requiring Adobe Reader 6.0. Dates for previous editions are not given. Annotation ©2004 Book News, Inc., Portland, OR

Product Details

ISBN-13: 9781627039307
Publisher: Springer-Verlag New York, LLC
Publication date: 11/27/2014
Series: Methods in Molecular Biology , #290
Edition description: 3rd ed. 2005
Pages: 371
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Value-Added eBook/PDAxii
1Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line1
2Detection of Mycoplasma Contaminations13
3Eradication of Mycoplasma Contaminations25
4Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting35
5Cytogenetic Analysis of Cell Lines51
6Human and Mouse Hematopoietic Colony-Forming Cell Assays71
7Isolation and Culture of Murine Macrophages91
8Isolation and Culture of Human Macrophages105
9Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture117
10In Vitro Generation of Lymphocytes From Embryonic Stem Cells135
11Hematopoietic Development of Human Embryonic Stem Cells in Culture149
12Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region163
13Culture of Human and Mouse Mesenchymal Cells173
14Isolation, Purification, and Cultivation of Murine and Human Keratinocytes187
15Isolation and Culture of Primary Human Hepatocytes207
16Primary Kidney Proximal Tubule Cells231
17Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity249
18Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue265
19Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells281
20Adult Ventricular Cardiomyocytes: Isolation and Culture305
21Isolation and Culture of Primary Endothelial Cells315
22Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions331
23Isolation and Characterization of Side Population Cells343
24Scalable Production of Embryonic Stem Cell-Derived Cells353

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