C. elegans: Methods and Applications

by Kevin Strange (Editor)

Overview

An Overview of C. elegans Biology Kevin Strange Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species Avril Coghlan, Jason E. Stajich and Todd W. Harris WormBase: Methods for Data Mining and Comparative Genomics Todd W. Harris and Lincoln D. Stein C. elegans Deletion Mutant Screening Robert J. Barstead and Donald G. Moerman Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes Jean-Louis Bessereau SNP Mapping M. Wayne Davis and Marc Hammarlund Creation of Transgenic Lines Using Microparticle Bombardment Methods Vida Praitis Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA Lisa Timmons Delivery Methods for RNAi in Caenorhabditis elegans Lisa Timmons Functional Genomic Approaches in C. elegans Todd Lamitina Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian Fluorescent Reporter Methods Harald Hutter Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans Michael M. Francis and Andres Villu Maricq Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis Michael A. Miller Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry Robby M. Weimer Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants Keith Nehrke Automated Imaging of C. elegans Behavior Christopher J. Cronin, Zhaoyang Feng and William R. Schafer Intracellular Ca2+ Imaging in Caehorhabditis elegans Rex A. Kerr and William R. Schafer In Vitro Culture of C. elegans Somatic Cells Kevin Strange and Rebecca Morrison Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS Flow Sorting System Rock Pulak et al.

Product Details

ISBN-13:	9781617376689
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/01/2010
Series:	Methods in Molecular Biology , #351
Edition description:	Softcover reprint of hardcover 1st ed. 2006
Pages:	292
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

An Overview of C. elegans Biology.- Comparative Genomics in C. elegans, C. briggsae, and Other Caenorhabditis Species.- WormBase.- C. elegans Deletion Mutant Screening.- Insertional Mutagenesis in C. elegans Using the Drosophila Transposon Mos1.- Single-Nucleotide Polymorphism Mapping.- Creation of Transgenic Lines Using Microparticle Bombardment Methods.- Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA.- Delivery Methods for RNA Interference in C. elegans.- Functional Genomic Approaches in C. elegans.- Assays for Toxicity Studies in C. elegans With Bt Crystal Proteins.- Fluorescent Reporter Methods.- Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans.- Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis.- Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocyhemistry.- Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants.- Automated Imaging of C. elegans Behavior.- Intracellular Ca2+ Imaging in C. elegans.- In Vitro Culture of C. elegans Somatic Cells.- Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS™ Flow-Sorting System.