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The International Symposium on "Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylenc" ,vas held in Agen, France from August 31 st and September 4th, 1992. The planning and management of the scientific and social programme of the Conference were carried out jointly by the "Ethylene Research Group" of ENSAlIN"P (Toulouse) and Agropole Congres Service (Agen). Since the last meetings in Israel (1984) and in Belgium (1988), ethylene physiology has gone through a period of exciting progress due to new developments in cellular and molecular bioiogy. New methods and tools have been developed to better understand the role and functions of ethylene in fruit ripening, flower senescence, abscission, piant growth, and cell differentiation. Genes involved in ethylene biosynthesis have been characterized and transgenic plants with altered ethylene production have been generated. The feasibility of delaying fruit ripening or flower senescence by genetic manipulation is now demonstrated, thus opening new perspectives for the postharvest handling of plant products. Some progress has also been made on the understanding of ethylene action. However, much remains to be done in this area to elucidate the ethylene signal transduction pathway. Around 140 scientists from 20 countries attended the Symposium. They presented 47 oral reports and 40 poster demonstrations. All of them are published in these proceedings. It has been a pleasure for us to organize this important Symposium and to edit this book.
|Series:||Current Plant Science and Biotechnology in Agriculture , #16|
|Edition description:||Softcover reprint of the original 1st ed. 1993|
|Product dimensions:||6.10(w) x 9.25(h) x (d)|
Table of ContentsEthylene Biosynthesis and Fruit Ripening.- Structural characteristics of ACC synthase isozymes and differential expression of their genes.- Monomeric and dimeric forms and mechanism-based inactivation of 1-aminocyclopropane-1-carboxylate synthase.- Characterization of the 1-aminocyclopropane- 1 -carboxylate (ACC) synthase isoenzymes (genes) in tomato.- Modifying fruit ripening by suppressing gene expression.- Cloning and expression analysis of an Arabidopsis thaliana 1-aminocyclopropane1-carboxylate synthase gene: pattern of temporal and spatial expression.- Relationship of ACC oxidase RNA, ACC synthase RNA and ethylene, in peach fruit.- Maximising the activity of the ethylene-forming enzyme.- Purification, characterization and subcellular localization of ACC oxidase from fruits.- Purification and characterization of ACC oxidase and its expression during ripening in apple fruit.- Mechanistic aspects of ACC oxidation to ethylene.- Apple ACC oxidase: Purification and characterization of the enzyme and cloning of its cDNA.- Biochemical and molecular characterization of ethylene-forming enzyme from avocado.- Identification of genes encoding EFE in tomato.- EFE multi-gene family in tomato plants: Expression and characterization.- Altered gene expression, leaf senescence, and fruit ripening by inhibiting ethylene synthesis with EFE-antisense genes.- Conversion of 1-aminocyclopropane- 1 -carboxylic acid to ethylene and its regulation by calcium in sunflower protoplasts.- Isolation of a ripening and wound-induced cDNA from Cucumis melo L. with homology to the ethylene-forming enzyme.- Isolation and characterisation of ethylene-forming enzyme genes from melon.- Immunocytolocalisation of ACC oxidase in tomato fruits.- Biochemical and immunocytological characterization of ACC oxidase in transgenic grape cells.- Effect of E8 protein on ethylene biosynthesis during tomato fruit ripening.- Expression of a bacterial ACC deaminase gene in tomato.- Stereospecific reaction of 1-aminocyclopropane-l-carboxylate deaminase.- Biochemical and molecular aspects of low oxygen action on fruit ripening.- Functional analysis of CX-Cellulase (endo-?-1,4-glucanase) gene expression in transgenic tomato fruit.- Inhibition of ethylene biosynthesis and suppression of cellulase and polygalacturonase in avocado fruit subjected to low oxygen storage.- Cold-induced climacteric rise of ethylene metabolism in Granny Smith apples.- Regulation by temperature of the propylene induced ethylene biosynthesis and ripening in “Hayward” kiwifruit.- Ethylene involvement in raspberry fruit ripening.- Effect of ethylene on sesquiterpene nootkatone production during the maturation-senescence stage in grapefruit (Citrus paradisi Macf).- Ethylene biosynthesis during the ripening of cherimoya (Annona cherimola, Mill).- Effects of CO2 on ethylene production by apples at low and high O2 concentrations.- High carbon dioxide treatment before storage as inducer or reducer of ethylene in apples.- ADH activity, via ethanol, affects ethylene production in tomato pericarp discs.- Two-dimensional protein patterns of cherimoya fruits during ripening.- Involvement of ethylene levels in delayed ripening of avocado cv. “Hass” at low temperature.- Relationship between polyamines and ethylene in cherimoya fruit ripening.- Modulation of gene expression under ethylene treatment in the latex of Hevea brasiliensis.- Immunodetection of ethylene-induced chlorophyllase from citrus fruit peel.- Ascorbate oxidase of Cucumis melo.- Ethylene Action.- Ethylene receptors.- Buckminsterfullerene (C60 buckyball) inhibition of ethylene release from senescing legume foliage and cut carnations.- Effect of diazocyclopentadiene (DACP) on cut carnations.- Reduced sensitivity to ethylene and delayed senescence in a group of related carnation cultivars.- In vitro study of ethylene binding sites in pea seedlings.- Stress Ethylene.- Fungal xylanase elicits ethylene biosynthesis and other defense responses in tobacco.- Stress ethylene in Hevea brasiliensis: Physiological, cellular and molecular aspects.- Wound ethylene synthesis in the stress-affected cells.- Ethylene in early signaling phenomena at the plant-microorganism interface.- Tomato ACC synthase: regulation of gene expression and importance of the C-terminal region in enzyme activity.- Regulation of ethylene synthesis in maize roots responses to stress.- Heavy metal induction of ethylene production and stress enzymes I. Kinetics of the responses.- Heavy metal induction of ethylene production and stress enzymes. II. Is ethylene involved in the signal transduction from stress perception to stress responses?.- Flooding resistance and ethylene. I. An ecophysiological approach with rumex as a model.- Flooding resistance and ethylene. II. Application of an advanced laser-driven photoacoustic technique in ethylene measurements on flooded rumex plants.- Flooding resistance and ethylene. III. The role of ethylene in shoot elongation of rumex plants in response to flooding.- Effect of saline stress on growth of 1ycopersiconesculentum plants and its relation with endogenous ethylene metabolism.- Ethylene biosynthesis in “Hayward” kiwifruit infected by Botrytis einerea.- Ethylene, stress and enzymatic activities in Hevea latex: the diversity of responses.- Molecular and physiological characterisation of the role of ethylene during pathogen attack of tomato fruit.- The use of antisense transgenic tomato plants to study the role of ethylene in responses to waterlogging.- Research on the diurnal courses of abscissic acid, 1-aminocyclopropane carboxylic acid and its malonyl conjugate contents in needles of damaged and undamaged spruces.- Flower Senescence. Abscission..- Hormonal and tissue-specific regulation of cellulase gene expression in abscission.- Changes in gene expression during leaf abscission.- Rapid ethylene-induced gene expression during petal abscission.- Abscission studies in a new mutant of navel oranges.- Ethylene regulation and function of flower senescence-related genes.- Cloning of ethylene biosynthetic genes involved in petal senescence of carnation and petunia, and their antisense expression in transgenic plants.- Interorgan regulation of post-pollination events in orchid flowers.- Roles of ethylene, ACC and short-chain saturated fatty acids in inter-organ communication during senescence of Cymbidium flowers.- The role of ethylene in the abscission and ripening of raspberry fruit Rubus idaeus cv Glen Clova.- Expression of two ACC synthase mRNAs in carnation flower parts during aging and following treatment with ethylene.- Promoting the activity of arginine decarboxylase and ornithine decarboxylase by ethylene and its significance to the control of abscission in citrus leaf explants.- Expression of EFE antisense RNA in tomato causes retardation of leaf senescence and most fruit ripening characteristics.- Growth, Development.- The role of ethylene in regulating growth of deepwater rice.- Gravity dependent ethylene action.- Ethylene and the growth of etiolated seedlings of lupinus albus L..- Various conditions of illumination and ethylene evolution.- Ethylene and vitrification of Fraxinus explants in vitro.- Stimulation of somatic embryogenesis in carrot by ethylene.- Relationship between ethylene and polyamine synthesis in plant regeneration.- Ethylene inhibits the morphogenesis of Vitis vinifera cuttings cultured in vitro.- Enhanced ethylene production by primary roots of Zea mays L. in response to sub -ambient partial pressures of oxygen.- Ethylene and phosphorylation of pea epicotyl proteins.- Knowledge of xylem sap flow rate is a pre-requisite for accurate estimates of hormone transport from roots to shoots.- Ethylene synthesis by fruit plants cultured in vitro.- Index of Authors.- Index of Keywords.