This book presents the latest advances concerning the regulation of chromosome segregation during cell division by means of centromeres and kinetochores. The authors cover both state-of-the-art techniques and a range of species and model systems, shedding new light on the molecular mechanisms controlling the transmission of genetic material between cell divisions and from parent to offspring.
The chapters cover five major areas related to the current study of centromeres and kinetochores: 1) their genetic and epigenetic features, 2) key breakthroughs at the molecular, proteomic, imaging and biochemical level, 3) the constitutive centromere proteins, 4) the role of centromere proteins in the physical process of chromosome segregation and its careful orchestration through elaborate regulation, and 5) intersections with reproductive biology, human health and disease, as well as chromosome evolution. The book offers an informative and provocative guide for newcomers as well as those already acquainted with the field.
Table of ContentsAkioshi, Bungo: Evolutionary lessons from species with unconventional centromeres and kinetochores; Bloom, Kerry: Centromere chromatin structure and the chromosome segregation mechanism; Dunleavy, Elaine: Centromere dynamics in the male and female germlines’; Erhardt, Silvia: Proposed to her "posttranslational modification of centromeric chromatin" but also discussing "role of non-coding RNA and transcription at the centromere"; Earnshaw, Bill: Lessons learned from proteomic efforts with mitotic chromosomes; Fachinetti, Dani: DNA sequences in centromere formation and function; Foltz, Dan: Orchestrating the specific assembly of centromeric nucleosomes; Freitag, Michael: Centromere function and heterochromatin in diverse fungi; Fukagawa, Tatsuo: Dynamic organization of CCAN proteins; Giulotto, Elena; The unique DNA sequences underlying equine centromeres; Grishchuk, Katya: Microtubule end-coupling at the kinetochore; Heun, Patrick: Artificial chromosomes and epigenetic centromere establishment; Jansen, Lars: Discoveries made at centromeres and kinetochores through quantitative microscopy; Kalitsis, Paul: The role of centromere defects in cancer; Lampson, Michael: Meiotic drive, asymmetric chromosome segregation, and determining what makes a centromere 'strong'; Miga, Karen: Centromere genomics; O'Neill, Rachel: Why transcribe centromeres?; Oliveira, Raquel: Release of centromere cohesion during mitosis; Saurin, Adrian: Spatiotemporal regulation of kinetochore signaling components; Straight, Aaron: The power of Xenopus extracts in the study of centromeres and kinetochores; Sullivan, Beth: Centromere Silencing Mechanism