Characterization Of S-Ribosylhomocysteinase (Luxs)

Characterization Of S-Ribosylhomocysteinase (Luxs)

by Jinge Zhu
ISBN-10:
3639165152
ISBN-13:
9783639165159
Pub. Date:
06/07/2009
Publisher:
VDM Verlag

Paperback

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Overview

Characterization Of S-Ribosylhomocysteinase (Luxs)

S-Ribosylhomocysteinase (LuxS) catalyzes the cleavage of the thioether bond in S-ribosylhomocysteine to produce L-
homocysteine and 4,5-dihydroxy-2,3-pentanedione,
the precursor of type II bacterial quorum sensing autoinducer.
This work carried out extensive mechanistic studies of the LuxS
reaction. The native metal cofactor of LuxS was identified as ferrous ion, instead of previously reported zinc ion, with a potential catalytic role. Substantial evidence was provided for the internal redox reaction, which comprised two consecutive carbonyl migration steps followed by β-elimination.
Three LuxS activity assays were developed and greatly facilitated the mechanistic investigations of LuxS. Two classes of
LuxS inhibitors were designed based on metal chelation and catalytic mechanism,
respectively. They encouraged future development of
LuxS inhibitors as novel antibacterial agents and helped probe the catalytic mechanism of LuxS.

Product Details

ISBN-13: 9783639165159
Publisher: VDM Verlag
Publication date: 06/07/2009
Pages: 224
Product dimensions: 0.51(w) x 6.00(h) x 9.00(d)

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