Chemical and Synthetic Approaches in Membrane Biology

Chemical and Synthetic Approaches in Membrane Biology

by Arun K. Shukla (Editor)

Paperback(Softcover reprint of the original 1st ed. 2017)

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Overview

Chemical and Synthetic Approaches in Membrane Biology by Arun K. Shukla

This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field.


Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes.

Product Details

ISBN-13: 9781493983131
Publisher: Springer New York
Publication date: 07/27/2018
Series: Springer Protocols Handbooks
Edition description: Softcover reprint of the original 1st ed. 2017
Pages: 270
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding


Desmond J. Hamilton, Yuheng Cai, Rupinder Kaur, Grant W. Marquart, Marilyn R. Mackiewicz, and Scott M. Reed



2. Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes


Josef Madl, Sarah Villringer, and Winfried Römer



3. Lipid Bilayer Patterns Fabrication by One-Photon Lithography


M. Florencia Sánchez and Dolores C. Carrer




4. Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels


Bharat Ramasubramanian Iyer, Ankit Gupta, and Radhakrishnan Mahalakshmi



5. Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy


Eliana S. Di Cairano, Paola Marciani, Stefania Moretti, and Carla Perego



6. Fluorescence-Based Screening of Membrane Proteins for Structural Studies



Jyoti Kumari, Surbhi Dhingra, and Janesh Kumar



7. Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins


Francisco J. Barrantes



8. Chemical Methods for Monitoring Protein Fatty-Acylation


Xiaoqiu Yuan and Howard C. Hang



9. Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research


Lucas B. Thal, Danielle M. Bailey, Oleg Kovtun, and Sandra J. Rosenthal



10. Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins


Serdar Uysal and Anthony Kossiakoff



11. Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis


Hidetoshi Kumagai and Yuichi Ikeda



12. Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies


Meaghan E. Ward, Vladimir Ladizhansky, and Leonid S. Brown

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