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Chlamydia trachomatis: Methods and Protocols

Chlamydia trachomatis: Methods and Protocols

by Amanda Claire Brown (Editor)

Hardcover(1st ed. 2019)

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Available for Pre-Order. This item will be available on August 28, 2019

Overview

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.

Product Details

ISBN-13: 9781493996933
Publisher: Springer New York
Publication date: 08/28/2019
Series: Methods in Molecular Biology , #2042
Edition description: 1st ed. 2019
Pages: 290
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA)


Kevin E. Eboigbodin and Mark Hoser



2. Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue


Naraporn Somboonna and Ilada Choopara



3. Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae


Kevin E. Eboigbodin



4. Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay


Kevin E. Eboigbodin



5. Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth


Tomislav Meštrović, Dezső P. Virok, Sunčanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, and Jasmina Vraneš



6. Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples



Amanda Claire Brown and Mette T. Christiansen



7. Multi Locus Sequence Typing (MLST) of Chlamydiales


Martina Jelocnik, Adam Polkinghorne, and Yvonne Pannekoek



8. Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method


Deborah C. Holt, Patiyan Andersson, Cameron Buckley, David M. Whiley, and Phillip M. Giffard



9. Dual RNA-Seq of Chlamydia and Host Cells


James W. Marsh, Regan Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, and Garry S.A. Myers




10. Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection


Oleg V. Podgorny, Nadezhda F. Polina, and Vassili N. Lazarev



11. Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions


Katerina Wolf, Mostafa Rahnama, and Kenneth A. Fields



12. Mutagenesis of Chlamydia trachomatis Using TargeTron



Mary M. Weber and Robert Faris



13. Forward and Reverse Genetic Analysis of Chlamydia


Mateusz Kędzior and Robert J. Bastidas




14. A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions


Rachel Ende and Isabelle Derré



15. Rodent Infections for Chlamydia spp.


Charles W. Armitage, Alison J. Carey, and Kenneth W. Beagley



16. Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy


Steven Liang and James B. Mahony



17. Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane


Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, and Elizabeth A. Rucks




18. High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis


Amanda Claire Brown and Nicole L. Kushner