Chloroplast Research in Arabidopsis: Methods and Protocols, Volume I

Chloroplast Research in Arabidopsis: Methods and Protocols, Volume I

by R. Paul Jarvis (Editor)


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Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Prools, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Prools serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Product Details

ISBN-13: 9781617792335
Publisher: Springer-Verlag New York, LLC
Publication date: 08/09/2011
Series: Methods in Molecular Biology , #774
Edition description: 2011
Pages: 374
Product dimensions: 7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Part I: Genetics, Cytology, and In Vivo Analysis

1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis
Enrique López-Juez and Alison Hills

2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy
Kevin Pyke

3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins
Stanislav Vitha and Katherine W. Osteryoung

4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts
Dong Wook Lee and Inhwan Hwang

5. Visualisation of Stromules on Arabidopsis Plastids
John C. Gray, James A. Sullivan, and Christine A. Newell

6. Analysis of Chloroplast Movement and Relocation in Arabidopsis
Masamitsu Wada and Sam-Geun Kong

7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots
John Stanga, Allison Strohm, and Patrick H. Masson

8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy
Stefan Hyman and R. Paul Jarvis

9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method
Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga

Part II: Gene Expression and Protein Accumulation

10. Isolation, Quantification, and Analysis of Chloroplast DNA
Beth A. Rowan and Arnold J. Bendich

11. Measurement of Transcription Rates in Arabidopsis Chloroplasts
Yan O. Zubo, Thomas Börner, and Karsten Liere

12. Studying the Structure and Processing of Chloroplast Transcripts
Alice Barkan

13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts
Toshiharu Shikanai and Kenji Okuda

14. Studying Translation in Arabidopsis Chloroplasts
Paolo Pesaresi

15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts
Lars L.E. Sjögren and Adrian K. Clarke

Part III: Protein Transport, Localization, and Topology

16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins
Sandra Tanz and Ian Small

17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays
Henrik Aronsson and R. Paul Jarvis

18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions
Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell

19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
Thomas Bals and Danja Schünemann

20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction
Chiung-Chih Chu and Hsou-min Li

21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases
John Froehlich

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