This volume offers a comprehensive update and overview of the field of cholinergic transmission as presented by some thirty distinguished investigators who were recruited for their task from Germany, Great Britain, Canada, USA, Sweden, Israel, France and Italy. Exciting new discoveries, described in this volume, are due to recent methodological breakthroughs. These discoveries throw new light on many areas of cholinergic mechanisms.
Table of ContentsList of contributors. Preface. The Otto Loewi Lecture. Loewi's discovery and the XXI century. Section I. Neurobiology of Cholinergic Transmission. 1. Molecular properties and cellular distribution of cholinergic synaptic proteins. 2. Redistribution of clathrin and synaptophysin at the frog neuromuscular junction triggered by nerve stimulation: immunocytochemical studies of vesicle trafficking. 3. Transcriptional regulation of the human choline acetyltransferase gene. 4. Biosynthesis and integration of acetylcholinesterase in the cholinergic synapse. Section II. Vesicular Acetylcholine Transporter. 5. The VAChT/ChAT "cholinergic gene locus": new aspects of genetic and vesicular regulation of cholinergic function. 6. Expression of the vesicular acetylcholine transporter in mammalian cells. 7. Interactions of protons with the acetylcholine transporter of synaptic vesicles. Section III. Neuronal Nicotinic Receptors. 8. Nicotinic receptors of the vertebrate CNS: introductory remarks. 9. Nicotinic acetylcholine receptors on hippocampal neurons: cell compartment specific expression and modulatory control of channel activity. 10. Structure and function of neuronal nicotinic acetylcholine receptors. Section IV. Muscarinic Receptors: 1. Structural aspects. 11. 3-Heteroarylquinuclidin-2-ene derivatives as muscarinic antagonists: synthesis, structure-activity relationships and molecular modelling. 12. Allosteric regulation of muscarinic receptors. 13. Molecular aspects of muscarinic receptor assembly and function. Section V. Muscarinic Receptors: 2. Mechanisms of Desensitization. 14. Regulation of muscarinic acetylcholine receptor expressionand function. 15. The role of G-protein coupled receptor kinases in the regulation of muscarinic cholinergic receptors. 16. Activation, cellular redistribution and enhanced degradation of the G proteins G