The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Table of ContentsAnalytical Methods:
M.C. Rose, Characterization of Human Tracheobronchial Mucin Glycoproteins.
M. Fukuda, Characterization of O-Linked Saccharides from Cell Surface Glycoproteins.
D.F. Smith and B.V. Torres, Lectin Affinity Chromatography of Glycolipids and Glycolipid-Derived Oligosaccharides.
A. Kobata and K. Yamashita, Affinity Chromatography of Oligosaccharides on E4-Phytohemagglutinin-Agarose Column.
D. Zopf, S. Ohlson, J. Dakour, W. Wang, and A. Lundblad, Analysis and Purification of Oligosaccharides by High-Performance Liquid Affinity Chromatography.
R.R. Townsend, M.R. Hardy, and Y.C. Lee, Separation of Oligosaccharides Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection.
M.R. Hardy, Monosaccharide Analysis of Glycoconjugates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection.
S.W. Whiteheart, A. Passaniti, J.S. Reichner, G.D. Holt, R.S. Haltiwanger, and G.W. Hart, Glycosyltransferase Probes.
G.N. Misevic, Immunoblotting and Immunobinding of Acidic Polysaccharides Separated by Gel Electrophoresis.
S. Pelkonen and J. Finne, Polyacrylamide Gel Electrophoresis of Capsular Polysaccharides of Bacteria.
V. Kolb-Bachofen, Carbohydrate Receptor Binding Using Colloidal Gold.
J. Dabrowski, Two-Dimensional Proton Magnetic Resonance Spectroscopy.
R.A. Laine, Tandem Mass Spectroscopy of Oligosaccharides.
C.L.M. Stults, C.C. Sweeley, and B.A. Macher, Glycosphingolipids: Structure, Biological Source, and Properties.
M. McNeil, D. Chatterjee, S.W. Hunter,and P.J. Brennan, Mycobacterial Glycolipids: Isolation, Structures, Antigenicity, and Synthesis of Neoantigens.
G.A. Nores, N. Hanai, S.B. Levery, H.L. Eaton, M.E.K. Salyan, and S.-i. Hakomori, Synthesis and Characterization of Ganglioside GM3 Derivatives: Lyso-GM3, De-N-Acetyl-GM3, and Other Compounds.
Y.C. Lee and R.T. Lee, Conjugation of Glycopeptides to Proteins.
R.T. Lee and Y.C. Lee, Conversion of Galactosyl Residues to 6-Amino Derivatives for Attachment of Functional Groups.
J. Amano and A. Kobata, Quantitative Conversion of Mucin-Type Sugar Chains to Radioactive Oligosaccharides.
D. Spillmann and J. Finne, Specific Labeling of Cell Surface Poly-N-acetyllactosamine Glycans.
E.S. Simon, E.J. Toone, G. Ostroff, M.D. Bednarski, and G.M. Whitesides, Preparation of Cytidine 5'-Monophospho-N-acetylneuraminic Acid and Uridine 5'-Diphosphoglucuronic Acid: Syntheses of ~ga-2, 6-Sialyllactosamine, ~ga-2, 6-Sialyllactose, and Hyaluronic Acid.
R.H. Quarles, Human Monoclonal Antibodies Associated with Neuropathy.
L.E. Silberstein, L.C. Jeffries, J. Goldman, and S.L. Spitalnik, Production of Carbohydrate-Specific Human Monoclonal Antibodiesin Vitro.
M. Li, J.J. Distler, and G.W. Jourdian, Phosphomannosyl Receptors from Bovine Testis.
T. Kawasaki, N. Kawasaki, and I. Yamashina, Mannose/N-Acetylglucosamine-Binding Proteins from Mammalian Sera.
T. Kawasaki, M.I. Yamashina and I. Yamashina, Galactose/N-Acetylgalactosamine-Binding Protein and Mannose/L-Fucose/N-Acetylglucosamine-Binding Proteins from Rat Peritoneal Macrophages.
H. Kaku and I.J. Goldstein, Snowdrop Lectin.
K. Yamashita, A. Kobata, T. Suzuki, and K. Umetsu, Allomyrina dichotoma Lectins.
M.E. Etzler, Vegetative Tissue Lectins from Dolichos Biflorus.
H. Schachter, I. Brockhausen, and E. Hull, High-Performance Liquid Chromatography Assays for N-Acetylglucosaminyltransferase Involved in N- and O-Glycan Synthesis.
N. Taniguchi, A. Nishikawa, S. Fujii, and J. Gu, Glycosyltransferase Assays Using Pyridylaminated Acceptors: N-Acetylglucosaminyltransferase III, IV, and V.
H.H. Higa, A. Manzi, S. Diaz, and A. Varki, Sialate 9-O-Acetylesterase from Rat Liver.
S. Diaz, H.H. Higa, and A. Varki, Glycoprotein Sialate 7(9)-O-Acetyltransferase from Rat Liver Golgi Vesicles.
G. Sugumaran, D.E. Humphries, and J.E. Silbert, Microsomal Sulfation of Proteochondroitin, Chondroitin, and Chondroitin Oligosaccharides.
D.E. Humphries, G. Sugumaran, and J.E. Silbert, Decreasing Sulfation of Proteoglycans Produced by Cultured Cells.
M. Yanagishita, A. Salustri, and V.C. Hascall, Specific Activity of Radiolabeled Hexosamines in Metabolic Labeling Experiments.
D.R.P. Tulsiani and O. Touster, Mannosidase IA from Rat Liver Golgi Membranes.
G.P. Kaushal and A.D. Elbein, Glycoprotein Processing Enzymes of Plants.
Y.-T. Li and S.-C. Li, Ceramide Glycanase from Leech, Hirudo medicinalis, and Earthworm, Lumbricus terrestris.
M. Ito and T. Yamagata, Endoglycoceramidase from Rhodococcus Species G-74-2.
T. Muramatsu, Endo-~gb-Galactosidase Releasing Gal(~ga1-3)Gal.
D.R.P. Tulsiani and O. Touster, Lysosomal ~ga-d-Mannosidase from Rat Epididymis.
S. Alexander and J.H. Elder, Endoglycosidases from Flavobacterium meningosepticum and Application to Biological Problems.
Y. Igarashi, H. Nojiri, N. Hanai, and S.-i. Hakomori, Gangliosides that Modulate Membrane Protein Function.
R.L. Schnaar, B.K. Brandley, L.K. Needham, P. Swank-Hill, and C.C. Blackburn, Adhesion of Eukaryotic Cells to Immobolized Carbohydrates.
T. Sasaki and A. Abe, Glycolipid Transfer Protein from Pig Brain.