ISBN-10:
0470528125
ISBN-13:
2900470528128
Pub. Date:
10/05/2010
Publisher:
Wiley
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications / Edition 6

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications / Edition 6

by R. Ian Freshney
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  • Product Details

    ISBN-13: 2900470528128
    Publisher: Wiley
    Publication date: 10/05/2010
    Edition description: Older Edition
    Pages: 796
    Product dimensions: 6.50(w) x 1.50(h) x 9.50(d)

    About the Author

    R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

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    Table of Contents

    List of Figures, xix

    List of Color Plates, xxiii

    List of Tables, xxv

    List of Protocols, xxvii

    List of Minireviews, xxix

    Preface and Acknlowledgments, xxxi

    Abbreviations, xxxiii

    About the Companion Website xxxviii

    1. Introduction, 1

    2. Biology of Cultured Cells, 19

    3. Laboratory Design and Layout, 37

    4. Equipment and Materials, 51

    5. Aseptic Technique, 73

    6. Safety, Bioethics, and Validation, 89

    7. Culture Vessels and Substrates, 111

    8. Defined Media and Supplements, 125

    9. Serum-Free Media, 149

    10. Preparation and Sterilization, 173

    10. Supplementary Material*

    11. Primary Culture, 207

    11. Supplementary Material*

    12. Subculture and Cell Lines, 235

    13. Authentication and Validation, 259

    14. Microbial Contamination, 289

    15. Cryopreservation and Banking, 307

    16. Cloning and Selection, 327

    16. Supplementary Material*

    17. Cell Sorting, 347

    18. Cell Line Characterization, 359

    19. Differentiation, 383

    20. Three-Dimensional Culture 401

    21. Scale-up and Automation, 427

    22. Senescence, Immortalization, and Transformation, 455

    23. Quantitation, 483

    23 Supplementary Material*

    24. Cytotoxicity, 513

    24. Supplementary Material*

    25. Culture of Specific Cell Types, 531

    25. Supplementary Material*

    26. Stem Cells, 571

    26. Supplementary Material*

    27. Training Programs, 591

    28. Problem Solving 627

    29. In Conclusion, 651

    Supplementary Material*

    Appendix III: Sources of Equipment and Material

    Appendix IV: Suppliers and Other Resource

    What People are Saying About This

    From the Publisher

    "The book's sixth incarnation includes new sections on organotypic culture and different cell and stem cell types". (Veterinary Times, 11 April 2011)

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