Pub. Date:
Cambridge University Press
Cytometric Analysis of Cell Phenotype and Function

Cytometric Analysis of Cell Phenotype and Function

by Desmond A. McCarthy, Marion G. MaceyDesmond A. McCarthy


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Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings due to improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding, and apoptosis. The volume guides the reader through data interpretation, quality control procedures, pitfalls, and problems.

Product Details

ISBN-13: 9780521660297
Publisher: Cambridge University Press
Publication date: 11/28/2001
Pages: 430
Product dimensions: 7.44(w) x 9.69(h) x 1.10(d)

Table of Contents

1. Principles of flow cytometry Marion G. Macey; 2. Introduction to the general principles of sample preparation Desmond A. McCarthy; 3. Fluorescence and fluorochromes Desmond A. McCarthy; 4. Quality control in flow cytometry David Barnett and John T. Reilly; 5. Data analysis in flow cytometry Mark W. Lowdell; 6. Laser scanning cytometry: application to the immunophenotyping of hematological malignancies Richard J. Clatch; 7. Leucocyte immunobiology Marion G. Macey and Timothy M. Milne; 8. Immunophenotypic analysis of leucocytes in disease Jamie D. Cavenagh and Marion G. Macey; 9. Analysis and isolation of minor cell populations Terry Hoy; 10. Cell cycle, DNA and DNA ploidy analysis Paul D. Allen and Adrian C. Newland; 11. Cell viability, necrosis and apoptosis Paul D. Allen and Adrian C. Newland; 12. Phagocyte biology and function Marion G. Macey; 13. Intracellular measures of signalling pathways James W. Jacobberger and David W. Hedley; 14. Cell-cell interactions Marion G. Macey and Mary R. Cahill; 15. Nucleic acids Charles L. Goolsby; 16. Microbial infections David Lloyd, David J. Mason and Marc T. E. Suller; 17. Leucocyte cell surface antigens Marion G. Macey; 18. Recent and future developments: conclusions Desmond A. McCarthy; Appendix 1. Abbreviations; Appendix 2. Useful Internet sites.

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