Derivative Spectrophotometry and PAM-Fluorescence in Comparative Biochemistry

by Vladimir S. Saakov, Alexander I. Krivchenko, Eugene V. Rozengart, Irina G. Danilova

Overview

This book presents various examples of how advanced fluorescence and spectroscopic analytical methods can be used in combination with computer data processing to address different biochemical questions. The main focus is on evolutionary biochemistry and the description of biochemical and metabolic issues; specifically, the use of pulse amplitude modulated fluorescence (PAM) for the functional analysis of the cellular state, as well as results obtained by means of the derivative spectroscopy method characterizing structural reorganization of a cell under the influence of external factors, are discussed. The topics presented here will be of interest to biologists, geneticists, biophysicists and biochemists, as well as experts in analytical chemistry, pharmaceutical chemistry and radio chemistry and radio activation studies with protonen and alpha-particles. It also offers a valuable resource for advanced undergraduate and graduate students in biological, physical and chemical disciplines whose work involves derivative spectrophotometry and PAM-fluorescence.

Product Details

ISBN-13:	9783319791722
Publisher:	Springer International Publishing
Publication date:	03/13/2019
Edition description:	Softcover reprint of the original 1st ed. 2015
Pages:	611
Product dimensions:	6.10(w) x 9.25(h) x (d)

Table of Contents

Introduction. Development of the methodological base, disputes, conclusions.- Successes of the pulse amplitude modulated fluorescence application.- Methodological approaches in our experimental work.- Application of Derivative Spectrophotometry in Comparative Biochemical Studies.- The Range of DHSO Application in Experiments with Pigments of Plants and Animals.- Conclusion.