Diagnosis of Sexually Transmitted Diseases: Methods and Protocols

Diagnosis of Sexually Transmitted Diseases: Methods and Protocols

Hardcover(2012)

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Product Details

ISBN-13: 9781617799365
Publisher: Springer-Verlag New York, LLC
Publication date: 07/12/2012
Series: Methods in Molecular Biology , #903
Edition description: 2012
Pages: 439
Product dimensions: 7.01(w) x 10.00(h) x 0.05(d)

Table of Contents

1. Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections

Fernando Vazquez, Santido Melón, Luis Otero, and María de Oña

2. Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots

Davide Gibellini, Elisa De Crignis, and Maria Carla Re

3. Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples

Tony Bruns and Katrin Steinmetzer

4. Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis

Linus Christerson and Björn Herrmann

5. Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens

Sin Hang Lee

6. The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease

Cheng-Yen Chen and Ronald C. Ballard

7. Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients

R. B. Takkenberg, S. Menting, and M.G.H.M. Beld

8. Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance Mediating Mutations in Region V of the 23S rRNA Gene

Jørgen Skov Jensen

9. Protocols for Detection and Typing of Treponema pallidum Using PCR Methods

S.M. Bruisten

10. Protocol for the Extraction of DNA from Dried Blood in the Diagnosis of Convenital CMV Infection

Jutte J.C. de Vries, Maria Barbi, Sandro Binda, and Eric C.J. Claas

11. Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis

Beatrice Vitali, Elena Biagi, and Patrizia Brigidi

12. Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis

Chung-Ter uhang and Shu-Ying Li

13. Protocol for the Detection and Genotyping of Human Papilloma viruses Using a Liquid Bead Microarray Assay (LBMA)

Stephen Cherne and Qinghua Feng

14. Protocol for the Use of Enzyme-Linked Hybridisation Assays for Genital Ulcer Disease

Seweryn Bialasiewicz, Ian M. Mackay, David M. Whiley, and Theo P. Sloots

15. Protocol for the Rapid Detection of the Urogential Tract Mollicutes and Chlamydia with Concomitant LGV –(sub)typing

Nadine Möbius, Wibke Brenneisen, Anke Schaeffer, and Birgit Henrich

16. Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV

Qian-Feng Xia

17. Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer

Chou-Pong Pau, Susan K. Wells, and Timothy C. Granade

18. Protocol for the Use of Light Upon Extension (LUX) Real-Time PCR for the Determination of Viral Load in HBV Infection

Guimin Li, Wangfeng Li, and Lixia Liu

19. Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations

Sang-Myung Lee, Kyo Seon Hwang, Sang Kyung Kim, and Tae Song Kim

20. Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens

Cheng-Yen Chen and Allan Pillay

21. Guidelines for the Use of Molecular Biological Methods to Detect Sexually Transmitted Pathogens in Cases of Suspected Sexual Abuse in Children

Margaret R. Hammerschlag and Charlotte A. Gaydos

22. Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae

Namraj Goire, Theo P. Sloots, Michael D. Nissen, and David M. Whiley

23. Protocol for a Facile Multiplex PCR for Multi-Antimicrobial Resistance and Gonococcus Detection

Ratana Lawung, Rungrot Cherdtrakulkiat, and Virapong Prachayasittikul

24. Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae

Lydgia A. Jackson and David W. Dyer

25. Protocol for the Detection of Human Leukocyte Antigen Variants and Sexually Transmitted Infections: From Genotyping to Immunoassays

Jianming Tang and Anju Bansal

26. Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-Parameter Flow Cytometry

Kathrin Schönberg, Maryam Hejazi, and Markus Uhrberg

27. Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity

Chitra Rajakuberan, Brett J. Hilton, and Roland Wolkowicz

28. Self Collection of Specimens for Nucleic Acid-Based Diagnosis of Pharyngeal, Cevicovaginal, Urethral and Rectal Neisseria gonorrhoeae and Chlamydia trachomatis Infections

Vivian Levy, Craig S. Blackmore, and Jeffrey D. Klausner

29. Sexually Transmitted Diseases – Reflections on Metaphors and Ethics

Gisela Badura-Lotter

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