ISBN-10:
0879696362
ISBN-13:
9780879696368
Pub. Date:
01/13/2003
Publisher:
Cold Spring Harbor Laboratory Press
DNA Science: A First Course in DNA Technology / Edition 2

DNA Science: A First Course in DNA Technology / Edition 2

by David H. Micklos, Greg A. Freyer

Hardcover

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Product Details

ISBN-13: 9780879696368
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 01/13/2003
Series: Textbook Series
Edition description: Subsequent
Pages: 592
Product dimensions: 8.78(w) x 11.20(h) x 1.31(d)

Table of Contents

Chapters
1. How We Learned That DNA Is the Genetic Material
2. How We Learned the Function of DNA
3. How We Learned How Genes Are Regulated
4. Basic Tools and Techniques of DNA Science
5. Methods for Finding and Expressing Important Genes
6. Modern Methods for Analyzing Whole Genomes
7. The DNA Science of Cancer
8. Applying DNA Science to Human Genetics
Labs
Lab Safety and Adherence to National Institutes of Health Guidelines
1. Measurements, Micropipetting, and Sterile Techniques
2. Bacterial Culture Techniques
3. DNA Restriction Analysis
4. Effects of DNA Methylation on Restriction
5. Rapid Colony Transformation of E. coli with Plasmid DNA
6. Assay for an Antibiotic Resistance Enzyme
7. Purification and Identification of Recombinant GFP
8. Purification and Identification of Plasmid DNA
9. Recombination of Antibiotic Resistance Genes
10. Transformation of E. coli with Recombinant DNA
11. Replica Plating to Identify Mixed E. coli Populations
12. Purification and Identification of Recombinant DNA
Answers to Discussion Questions
Appendices
1. Equipment, Supplies, and Reagents
2. Recipes for Media, Reagents, and Stock Solutions
3. Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage l
4. Cautions

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