DNA Sequencing Protocols

DNA Sequencing Protocols

Paperback(2nd ed. 2001)

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Product Details

ISBN-13: 9781489942128
Publisher: Springer-Verlag New York, LLC
Publication date: 09/01/2013
Series: Methods in Molecular Biology , #167
Edition description: 2nd ed. 2001
Pages: 244
Product dimensions: 6.10(w) x 9.25(h) x 0.02(d)

Table of Contents

Prefacev
Contributorsix
1Introduction to DNA Sequencing1
2The Universal Primers and the Shotgun DNA Sequencing Method13
3M13 Sequencing33
4Primer Design and Primer-Directed Sequencing39
5Direct Sequencing of DNA Produced in a Polymerase Chain Reaction53
6Solid Phase Fluorescent Sequencing of the CFTR Gene63
7Shotgun DNA Sequencing89
8Cycle Sequencing101
9Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers113
10Automated Fluorescent DNA Sequencing on the ABI PRISM 377119
11Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer153
12Fluorescent Sequencing Protocols for the ALF171
13Fluorescent Sequencing for Heterozygote Mutation Detection193
14Sequence Databases and the Internet215
15DNA Sequencing by Capillary Array Electrophoresis225
Index241

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